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Comparison of baseline cataract rates in AB and TL wildtype zebrafish strains Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-23 Bibek Khatiwada, Johanna L. Jones, Duran Zhao, Robert J. Gasperini, Bennet J. McComish, Kathryn P. Burdon
Zebrafish are an outstanding model for assessing the involvement of genes in paediatric cataracts. Gene discovery for cataracts is enhanced by manipulation of the genome of zebrafish embryos and comparing the phenotypes of mutant progeny with the wildtype embryos. However, wildtype laboratory fish can also develop cataracts, potentially confounding the results. In this study, we compared the baseline
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Chronic sleep deprivation impairs retinal circadian transcriptome and visual function Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-20 Shenzhen Huang, Wenxiao Zhang, Shuting Xuan, Hongli Si, Duliurui Huang, Mengru Ba, Di Qi, Xiaoting Pei, Dingli Lu, Zhijie Li
Sleep loss is common in modern society and is increasingly associated with eye diseases. However, the precise effects of sleep loss on retinal structure and function, particularly on the retinal circadian system, remain largely unexplored. This study investigates these effects using a chronic sleep deprivation (CSD) model in mice. Our investigation reveals that CSD significantly alters the retinal
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Targeting MicroRNA in myopia: Current insights Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-18 Zihao Zhuang, Licheng Li, Yang Yu, Xuemei Su, Shu Lin, Jianmin Hu
Myopia, the most prevalent eye condition, has sparked notable interest regarding its origin and prevention. MicroRNAs (miRNAs) are short, non-coding RNA strands typically consisting of 18–24 nucleotides. They play a central role in post-transcriptional gene regulation and are closely associated with both normal and pathological processes in organisms. Recent advances in next-generation sequencing and
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Aqueous humor as eye lymph: A crossroad between venous and lymphatic system Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-18 Vincenzo Benagiano, Anna Rizzi, Carmela Sannace, Giovanni Alessio, Domenico Ribatti, Rosanna Dammacco
Aqueous humor (AQH) is a transparent fluid with characteristics similar to those of the interstitial fluid, which fills the eyeball posterior and anterior chambers and circulates in them from the sites of production to those of drainage. The AQH volume and pressure homeostasis is essential for the trophism of the ocular avascular tissues and their normal structure and function. Different AQH outflow
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Dexamethasone sodium phosphate loaded nanoparticles for prevention of nitrogen mustard induced corneal injury Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-18 Sagun Poudel, Ehsan Kaffash, Long Zhao, Rudra Pangeni, Woon Nam Chow, Qingguo Xu
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Intraocular mRNA delivery with endogenous MmPEG10-based virus-like particles Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-16 Mengke Li, Zhong Liu, Dongliang Wang, Jinguo Ye, Zhuoxing Shi, Caineng Pan, Qikai Zhang, Rong Ju, Yingfeng Zheng, Yizhi Liu
Virus-like particles (VLP) are a promising tool for intracellular gene delivery, yet their potential in ocular gene therapy remains underexplored. In this study, we bridged this knowledge gap by demonstrating the successful generation and application of vesicular stomatitis virus glycoprotein (VSVG)-pseudotyped mouse PEG10 (MmPEG10)-VLP for intraocular mRNA delivery. Our findings revealed that PEG10-VLP
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Examining the correlation of lymphangiogenesis biomarkers with clinical condition in Age-Related Macular Degeneration (AMD) Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-13 Bagim Aycin Cakir Ince, Murat Kucukevcilioglu, Cigdem Yucel, Ali Hakan Durukan
The aim of this study is to investigate the relationship between age-related macular degeneration (AMD) and lymphangiogenesis biomarkers, namely LYVE-1, Podoplanin, VEGF-C, VEGFR-2 and VEGFR-3. This prospective and interventional study includes 30 patients with AMD which may be dry or wet type and 30 controls for whom vitrectomy and phacoemulsification was indicated due to additional pathologies (epiretinal
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Possible retinotoxicity of long-term vardenafil treatment Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-13 Klaudia Szabó, Bulcsú Dékány, Anna Énzsöly, Rozina Ida Hajdú, Lenke Kornélia Laurik-Feuerstein, Arnold Szabó, Tamás Radovits, Csaba Mátyás, Attila Oláh, Krisztián András Kovács, Ágoston Szél, Gábor Márk Somfai, Ákos Lukáts
Phosphodiesterase (PDE) inhibitors – such as vardenafil – are used primarily for treating erectile dysfunction via increasing cyclic guanosine monophosphate (cGMP) levels. Recent studies have also demonstrated their significant cardioprotective effects in several diseases, including diabetes, upon long-term, continuous application. However, PDE inhibitors are not specific for PDE5 and also inhibit
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Aqueous humour protein dysregulation in Asian eyes with primary open angle glaucoma Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-10 Darren Mun Yoong Choy, Bryan Chin Hou Ang, Sunil S. Adav, Yu Bo Zheng, Caius Jun Shyan Goh, Jin Wei, Arjunan Kumaran, Chun Hau Chua, Nicola Yian Gan, Siu Kwan Sze, Leonard Wei Leon Yip
The pathophysiology of Primary Open Angle Glaucoma (POAG) remains poorly understood. Through proteomic analysis of aqueous humour (AH) from POAG patients, we aim to identify changes in protein composition of these samples compared to control samples.
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Autophagy in dry AMD: A promising therapeutic strategy for retinal pigment epithelial cell damage Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-07 Zhao Zhang, Fengming Liang, Jun Chang, Xiaoqian Shan, Zhixian Yin, Li Wang, Shujiao Li
Dry age-related macular degeneration (AMD) is a prevalent clinical condition that leads to permanent damage to central vision and poses a significant threat to patients' visual health. Although the pathogenesis of dry AMD remains unclear, there is consensus on the role of retinal pigment epithelium (RPE) damage. Oxidative stress and chronic inflammation are major contributors to RPE cell damage, and
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Increased molar ratio of free fatty acids to albumin in blood as cause and early biomarker for the development of cataracts and Alzheimer's disease Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-06 Dietmar Glaesser, Martin Iwig
Cataracts and Alzheimer's disease (AD) are closely linked and are associated with aging and with systemic diseases that increase the molar ratio of free fatty acids to albumin (mFAR) in the blood. From the results of our earlier studies on the development of senile cataracts and from results recently published in the literature on the pathogenesis of Alzheimer's disease, we suggest that there is a
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INOS ablation promotes corneal wound healing via activation of Akt signaling Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-05 Ziwen Sun, Kunpeng Lu, Qing He, Yang Tang, Haoru Li, Emmanuel Eric Pazo, Lizhi Hu, Ruihua Wei
Corneal injury leads to impaired normal structure of the cornea. Improving the wound healing process in epithelial cells significantly contributes to ocular damage treatments. Here, we aimed to investigate the potential mechanisms of nitric oxide (NO) and its mediator, inducible nitric oxide synthase (iNOS), in the process of corneal wound healing. We established a corneal injury model of iNOS mice
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Validation of retinal oximetry vessel selection using fluorescein angiography in patients with optic disc drusen Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-04 Mia Uhre Guldfeldt, Freja Pind Pilegaard, Lasse Malmqvist, Oliver Niels Klefter, Steffen Hamann
Retinal oximetry could provide insights into the pathophysiology of optic nerve disease, including optic disc drusen (ODD). Vessel selection for oximetry analysis is based on morphological characteristics of arterioles and venules and supported by an overlay of estimated blood oxygen saturations. The purpose of this cross-sectional study was to determine the validity of this vessel selection procedure
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Retinal microcirculation: A window into systemic circulation and metabolic disease Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-03 Yue Yuan, Meiyuan Dong, Song Wen, Xinlu Yuan, Ligang Zhou
The retinal microcirculation system constitutes a unique terminal vessel bed of the systemic circulation, and its perfusion status is directly associated with the neural function of the retina. This vascular network, essential for nourishing various layers of the retina, comprises two primary microcirculation systems: the retinal microcirculation and the choroidal microcirculation, with each system
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Tamoxifen protects photoreceptors in the sodium iodate model Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-01 Timothy T. Lee, Brent A. Bell, Brandon D. Anderson, Ying Song, Joshua L. Dunaief
Because the selective estrogen receptor modulator tamoxifen was shown to be retina-protective in the light damage and rd10 models of retinal degeneration, the purpose of this study was to test whether tamoxifen is retina-protective in a model where retinal pigment epithelium (RPE) toxicity appears to be the primary insult: the sodium iodate (NaIO) model. C57Bl/6J mice were given oral tamoxifen (in
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Two-phase mechanism in the treatment of corneal stromal fibrosis with topical losartan Exp. Eye Res. (IF 3.4) Pub Date : 2024-04-01 Steven E. Wilson
Recent studies in rabbits and case reports in humans have demonstrated the efficacy of topical losartan in the treatment of corneal scarring fibrosis after a wide range of injuries, including chemical burns, infections, surgical complications, and some diseases. It is hypothesized that the effect of losartan on the fibrotic corneal stroma occurs through a two-phase process in which losartan first triggers
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Nicotinamide promotes the differentiation of functional corneal endothelial cells from human embryonic stem cells Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-30 Dulei Zou, Ting Wang, Wenjing Li, Xin Wang, Bochao Ma, Xiangyue Hu, Qingjun Zhou, Zongyi Li, Weiyun Shi, Haoyun Duan
Corneal transplantation represents the primary therapeutic approach for managing corneal endothelial dysfunction, but corneal donors remain scarce. Anterior chamber cell injection emerges as a highly promising alternative strategy for corneal transplantation, with pluripotent stem cells (PSC) demonstrating considerable potential as an optimal cell source. Nevertheless, only a few studies have explored
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Analysis aqueous humor lipid profile of neovascular glaucoma secondary to diabetic retinopathy and lipidomic alteration response to anti-VEGF treatment Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-29 Xiaoyun Wang, Xiaohua Ma, Jingyao Song, Bing Liu, Jiawei Wang
The objective of this study was to examine the lipid spectrum of aqueous humor (AH) in patients with neovascular glaucoma (NVG) secondary to proliferative diabetic retinopathy and to investigate the lipid alteration response to anti-vascular endothelial growth factor (anti-VEGF) treatment. Lipidomic analysis using ultra-high performance liquid chromatography–tandem mass spectrometry was conducted to
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Optimizing retinal ganglion cell nuclear staining for automated cell counting Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-28 Fangyu Lin, Su-Ting Lin, Jiaxing Wang, Eldon E. Geisert
The retinal ganglion cells (RGCs) serve as the critical pathway for transmitting visual information from the retina to the brain, yet they can be dramatically impacted by diseases such as glaucoma. When investigating disease processes affecting RGCs in mouse models, accurately quantifying affected cells becomes essential. However, the use of pan RGC markers like RBPMS or THY1 presents challenges in
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Fibronectin binds integrin α5β1 to regulate macular neovascularization through the Wnt/β-catenin signaling pathway Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-27 Xiaoran Zhao, Liting Hu, Guibo Liu, Xiaoni Yin, Qingyun Gong, Ying Li, Qinghua Li, Yuzheng Zhou, Yibin Sun, Chunyan Guo, Zhaodong Du
Age-related macular degeneration (AMD) is a progressive, degenerative disease of the macula. The formation of macular neovascularization (MNV) and subretinal fibrosis of AMD is the most classic cause of the loss of vision in older adults worldwide. While the underlying causes of MNV and subretinal fibrosis remain elusive, the common feature of many common retinal diseases is changes the proportions
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Local administration of myeloid-derived suppressor cells prevents progression of immune-mediated dry eye disease Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-26 Pier Luigi Surico, Seokjoo Lee, Rohan Bir Singh, Amirreza Naderi, Shilpy Bhullar, Tomas Blanco, Yihe Chen, Reza Dana
Myeloid derived suppressor cells (MDSCs) are a heterogenous population of immature hematopoietic precursors with known immunoregulatory functions. The immunosuppressive role of MDSCs has been highlighted in several inflammatory ophthalmic disorders; however, their therapeutic application in suppressing the immune-mediated changes in dry eye disease (DED) has not been studied. We observed significant
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Single-cell profiling transcriptomic reveals cellular heterogeneity and cellular crosstalk in choroidal neovascularization model Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-25 Ming Tong, Yun Bai, Xiaoyan Han, Lingjie Kong, Ling Ren, Linyu Zhang, Xiumiao Li, Jin Yao, Biao Yan
Choroidal neovascularization (CNV) is a hallmark of neovascular age-related macular degeneration (nAMD) and a major contributor to vision loss in nAMD cases. However, the identification of specific cell types associated with nAMD remains challenging. Herein, we performed single-cell sequencing to comprehensively explore the cellular diversity and understand the foundational components of the retinal
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PEDF-derived peptide protects against Amyloid-β toxicity in vitro and prevents retinal dysfunction in rats Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-23 Amanda Qarawani, Efrat Naaman, Rony Ben-Zvi Elimelech, Michal Harel, Chen Itzkovich, Shadi Safuri, Nitsan Dahan, Jack Henkin, Shiri Zayit-Soudry
Amyloid-beta (Aβ), a family of aggregation-prone and neurotoxic peptides, has been implicated in the pathophysiology of age-related macular degeneration (AMD). We have previously shown that oligomeric and fibrillar species of Aβ42 exerted retinal toxicity in rats, but while the consequences of exposure to amyloid were related to intracellular effects, the mechanism of Aβ42 internalization in the retina
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Photoreceptor deficits appear at eye opening in Rs1 mutant mouse models of X-linked retinoschisis Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-19 Matthew J. Tarchick, Craig Beight, Paul B. Bonezzi, Neal S. Peachey, Jordan M. Renna
X-linked retinoschisis (XLRS) is an early onset degenerative retinal disease characterized by cystic lesions in the middle layers of the retina. These structural changes are accompanied by a loss of visual acuity and decreased contrast sensitivity. XLRS is caused by mutations in the gene which encodes the secreted protein Retinoschisin 1. Young -mutant mouse models develop key hallmarks of XLRS including
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Role of mRNA-binding proteins in retinal neovascularization Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-19 Pei Lin, Wenye Cao, Xuemei Chen, Ningzhi Zhang, Yiqiao Xing, Ning Yang
Retinal neovascularization (RNV) is a pathological process that primarily occurs in diabetic retinopathy, retinopathy of prematurity, and retinal vein occlusion. It is a common yet debilitating clinical condition that culminates in blindness. Urgent efforts are required to explore more efficient and less limiting therapeutic strategies. Key RNA-binding proteins (RBPs), crucial for post-transcriptional
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Mesenchymal stem cell-based adjunctive therapy for Pseudomonas aeruginosa-induced keratitis: A proof-of-concept in-vitro study Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-15 Sreya Mitra, Vasudeva Tati, Prabhudatta Das, Joveeta Joseph, Bhupesh Bagga, Sachin Shukla
-induced keratitis is one of the most severe and challenging forms of corneal infection, owing to its associated intense inflammatory reactions leading to corneal necrosis and dense corneal scar with loss of vision. Since mesenchymal stem cells (MSCs) are reported to possess antimicrobial and immunomodulatory properties, they can be tested as an adjuvant treatment along with the antibiotics which are
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Screening and evaluation of antioxidants for retinal pigment epithelial cell protection: L-ergothioneine as a novel therapeutic candidate through NRF2 activation Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-13 Sijie Gu, Siqi Wu, Zesong Lin, Zhuo Han, Kunlun Mo, Huaxing Huang, Mingsen Li, Gen Li, Hong Ouyang, Li Wang
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Drp1-dependent mitochondrial fragmentation mediates photoreceptor abnormalities in type 1 diabetic retina Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-11 Shuyu Tang, Mengling Huang, Ruixuan Wang, Ming Li, Ning Dong, Ronghan Wu, Zailong Chi, Ling Gao
Recent studies have highlighted that retinal neurodegeneration precedes microvascular changes in diabetic retinopathy (DR), but the specific mechanisms remain unclear. Given the pivotal role of dysfunctional mitochondria and oxidative stress in early DR, our objective was to observe mitochondria-related alterations in the neural retina of type one diabetic mellitus mice with no evidence of DR (T1DM-NDR)
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Optical development in the murine eye lens of accelerated senescence-prone SAMP8 and senescence-resistant SAMR1 strains Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-11 Kehao Wang, Yutian Pu, Leran Chen, Masato Hoshino, Kentaro Uesugi, Naoto Yagi, Xiaoyong Chen, Yuu Usui, Akira Hanashima, Ken Hashimoto, Satoshi Mohri, Barbara K. Pierscionek
The eye lens is responsible for focusing objects at various distances onto the retina and its refractive power is determined by its surface curvature as well as its internal gradient refractive index (GRIN). The lens continues to grow with age resulting in changes to the shape and to the GRIN profile. The present study aims to investigate how the ageing process may influence lens optical development
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Rho-kinase inhibitor alleviates CD4+T cell mediated corneal graft rejection by modulating its STAT3 and STAT5 activation Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-11 Shang Li, Peng Zhang, Ao Li, Jiayu Bao, Zhiqiang Pan, Ying Jie
Penetrating keratoplasty remains the most common treatment to restore vision for corneal diseases. Immune rejection after corneal transplantation is one of the major causes of graft failure. In recent years, Rho-associated protein kinase (ROCK) inhibitors have been found to be associated with the activation of the STATs pathway and are widely studied in autoimmune diseases. Therefore, it may be possible
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Modeling autosomal dominant retinitis pigmentosa by using patient-specific retinal organoids with a class-3 RHO mutation Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-11 Xiao Lin, Zhuo-Lin Liu, Xiao Zhang, Wen Wang, Zhi-Qin Huang, Shu-Ning Sun, Zi-Bing Jin
Rhodopsin-mediated autosomal dominant retinitis pigmentosa (RHO-adRP) causes progressive vision loss and is potentially incurable, accounting for 25% of adRP cases. Studies on RHO-adRP mechanism were at large based on the biochemical and cellular properties, especially class-3. Nonetheless, the absence of an appropriate model for class-3 RHO-adRP has impeded comprehensive exploration. Here, induced
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Stop codon variant in EFEMP1 is associated with primary open-angle glaucoma due to impaired regulation of aqueous humor outflow Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-11 Junkai Tan, Suping Cai, Xiaolin Luo, Qiang Li, Yanbing Chen, Zijie Chen, Yukai Mao, Guo Liu, Mingming Yang, Xuyang Liu
It is known that the actin cytoskeleton and its associated cellular interactions in the trabecular meshwork (TM) and juxtacanalicular tissues mainly contribute to the formation of resistance to aqueous outflow of the eye. Fibulin-3, encoded by gene, has a role in extracellular matrix (ECM) modulation, and interacts with enzymatic ECM regulators, but the effects of fibulin-3 on TM cells has not been
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Sphingolipid biosynthetic inhibitor L-Cycloserine prevents oxidative-stress-mediated death in an in vitro model of photoreceptor-derived 661W cells Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-08 Faiza Tahia, Sandip K. Basu, Amanda Prislovsky, Koushik Mondal, Dejian Ma, Harry Kochat, Kennard Brown, Daniel J. Stephenson, Charles E. Chalfant, Nawajes Mandal
Oxidative stress plays a pivotal role in the pathogenesis of several neurodegenerative diseases. Retinal degeneration causes irreversible death of photoreceptor cells, ultimately leading to vision loss. Under oxidative stress, the synthesis of bioactive sphingolipid ceramide increases, triggering apoptosis in photoreceptor cells and leading to their death. This study investigates the effect of L-Cycloserine
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Intraocular pressure across the lifespan of Tg-MYOCY437H mice Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-05 Xiaoyan Zhang, Gaiping Xi, Pengchao Feng, Cong Li, Markus H. Kuehn, Wei Zhu
Transgenic C57BL/6 mice expressing human myocilin (Tg-MYOC) are a well-established model for primary open-angle glaucoma (POAG). While the reduced trabecular meshwork (TM) cellularity due to severe endoplasmic reticulum (ER) stress has been characterized as the etiology of this model, there is a limited understanding of how glaucomatous phenotypes evolve over the lifespan of Tg-Myoc mice. In this study
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Analysis of the mucosal chemokines CCL28, CXCL14, and CXCL17 in dry eye disease: An in vitro and clinical investigation Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-05 Alfredo Domínguez-López, Marta Blanco-Vázquez, Andrés Ángel Calderón-García, Carmen García-Vázquez, María J. González-García, Margarita Calonge, Amalia Enríquez-de-Salamanca
Mucosal chemokines have antimicrobial properties and play an important role in mucosal immunity. However, little is known about their expression on the ocular surface. This study aimed to analyze the expression of the mucosal chemokines CCL28, CXCL14 and CXCL17 in corneal and conjunctival epithelial cells under dry eye (DE) conditions, and in conjunctival samples from healthy subjects and DE patients
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Targeting mechanics-induced trabecular meshwork dysfunction through YAP-TGFβ Ameliorates high myopia-induced ocular hypertension Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-05 Qianwen Bu, Hai Zhu, Guangliang Cao, Ganyu Gong, Ying Su, Qingshu Ge, Wei Zhu, Zongyi Li, Xiaojing Pan
High myopia is a risk factor for primary open angle glaucoma (POAG). The pathological mechanism of high myopia induced POAG occurrence is not fully understood. In this study, we successfully established the guinea pig model of ocular hypertension with high myopia, and demonstrated the susceptibility of high myopia for the occurrence of microbead-induced glaucoma compared with non-myopia group and the
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Oleic acid induces lipogenesis and NLRP3 inflammasome activation in organotypic mouse meibomian gland and human meibomian gland epithelial cells Exp. Eye Res. (IF 3.4) Pub Date : 2024-03-05 Ren Liu, Mengqian Tu, Jianwen Xue, Bing Xiao, Jing Li, Lingyi Liang
The accumulation of oleic acid (OA) in the meibum from patients with meibomian gland dysfunction (MGD) suggests that it may contribute to meibomian gland (MG) functional disorder, as it is a potent stimulator of acne-related lipogenesis and inflammation in sebaceous gland. Therefore, we investigate whether OA induces lipogenesis and inflammasome activation in organotypic cultured mouse MG and human
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Characterization of lncRNA and mRNA profiles in ciliary body in experimental myopia Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-29 Shanshan Wu, Jiawen Hao, Dadong Guo, Zhongyu Ma, Qiuxin Wu, Ming Zhang, Hongsheng Bi
Currently, researchers have mainly focused on the role of the tissues of the posterior segment of the eyes in the development of myopia. However, the ciliary body, an anterior ocular tissue that contracts to initiate the process of accommodation, may also play an important role in the progression of myopia due to the increased demand for near work. In the present study, we established a lens-induced
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A minimally invasive method for diagnosing diseases of the eye Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-28 Vera B. Morhenn, Jonathan N. Mansbridge, Harvey Fishman
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Causal associations of refractive error and early age-related macular degeneration: A Mendelian randomization study Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-27 Lingling Wang, Wenlong Wei, YongJi Zhao, Sixi Chen, Dongjing Wu, Mengjun Tu
This study aims to determine the risk associated with early age-related macular degeneration (AMD) due to refractive errors (RE) using an analysis of genome-wide association study (GWAS) data through the two-sample Mendelian randomization approach. Single-nucleotide polymorphisms (SNPs) linked to refractive errors (RE) were obtained from numerous GWAS studies involving individuals of European descent
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Detection of retinal dysfunction induced by HCN channel inhibitors using multistep light stimulus and long-duration light stimulus ERG in rats Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-23 Naohisa Umeya, Yuki Yoshizawa, Kosuke Fukuda, Keigo Ikeda, Mami Kamada, Hiroshi Inada, Toru Usui, Izuru Miyawaki
Ivabradine, a hyperpolarization-activated cyclic nucleotide–gated (HCN) channel inhibitor, has been reported to induce photosensitivity-related visual disturbances such as phosphene in humans. Ivabradine-induced visual disturbances are caused by inhibition of HCN channels in the retina, and the mechanisms have been verified using HCN channel knockout mice and electroretinography (ERG). However, in
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Extracellular vesicle biomarkers in ocular fluids associated with ophthalmic diseases Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-22 Thomas Michael Shiju, Alex Yuan
Extracellular vesicles (EVs) are released as highly stable lipid bilayer particles carrying proteins, lipids, glycans and miRNAs. The contents of EVs vary based on the cellular origin, biogenesis route and the functional state of the cell suggesting certain diseased conditions. A growing body of evidence show that EVs carry important molecules implicated in the development and progression of ophthalmic
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Isolation and characterization of rabbit limbal niche cells Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-22 Guanyu Su, Xiaojie Guo, Lingjuan Xu, Bihui Jin, Yongyao Tan, Xiao Zhou, Wei Wang, Xinyu Li, Shusheng Wang, Guigang Li
Limbal niche cells (LNCs) are one of the most important supporting cells for corneal epithelial stem cells (CES), however, research on LNCs has been mostly limited to humans and rats previously. To expand the research work into the rabbit animal model, one of the most often used animals in stem cell study, this study was carried out for the isolation and identification of rabbit LNCs.
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TGF-β2-induced alterations of m6A methylation in hTERT RPE-1 cells Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-21 Xue Li, Xueru Zhao, Ruijie Yin, Min Yuan, Yongya Zhang, Xiaohua Li
N6-methyladenosine (m6A) is a major type of RNA modification implicated in various pathophysiological processes. Transforming growth factor β2 (TGF-β2) induces epithelial-mesenchymal transition (EMT) in retinal pigmental epithelial (RPE) cells and promotes the progression of proliferative vitreoretinopathy (PVR). However, the role of m6A methylation in the EMT of human telomerase reverse transcriptase
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Therapeutic effect and mechanism of action of pterostilbene nano drugs in dry eye models Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-20 Kexin Li, Meng Lin, Kaiyan Huang, Jiaxin Han, Linzhi Wei, Lijie Miao, Huijuan Chen, Qianwen Gong, Xingyi Li, Liang Hu
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Human lens epithelial-secreted exosomes attenuate ocular angiogenesis via inhibiting microglial activation Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-20 Yan Wu, Jiagui Wang, Ting Pan, Jie Lei, Yuanyuan Fan, Jingfan Wang, Changlin Xu, Qinyuan Gu, Xingxing Wang, Tianhao Xiao, Qinghuai Liu, Ping Xie, Zizhong Hu
The lens is an avascular tissue, where epithelial cells (LECs) are the primary living cells. The role of LECs-derived exosomes (LEC-exos) is largely unknown. In our study, we determined the anti-angiogenic role of LEC-exos, manifested as regressed retinal neovascularization (NV) using the oxygen-induced retinopathy (OIR), and reduced choroidal NV size and pathological vascular leakage using the laser-induced
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The response of retinal ganglion cells to optical defocused visual stimuli in mouse retinas Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-19 Chunghim So, Ting Zhang, Qin Wang, Chunting Qiu, De Lestrange-Anginieur Elie, Feng Pan
Myopia and astigmatism are two primary types of refractive errors characterized by inaccurate focusing images on the retina. This study aimed to investigate the response characteristics of Retinal Ganglion Cells (RGCs), represented by alpha (α) RGCs, when exposed to focused, simulated spherically defocused images and astigmatically defocused images projected onto mouse retinas.
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Long noncoding RNA ZFAS1: A novel anti-apoptotic target in Fuchs endothelial corneal dystrophy Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-17 Jini Qiu, Ruiping Gu, Qian Shi, Xueling Zhang, Jiayu Gu, Jun Xiang, Jianjiang Xu, Yujing Yang, Kun Shan
Fuchs endothelial corneal dystrophy (FECD) is the leading cause of endothelial keratoplasty without efficacious drug treatment. Recent studies have emphasized the involvement of epigenetic regulation in FECD development. Long non-coding RNAs (lncRNAs) are recognized as crucial epigenetic regulators in diverse cellular processes and ocular diseases. In this study, we revealed the expression patterns
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Influence of the calcium voltage-gated channel auxiliary subunit (CACNA2D1) absence on intraocular pressure in mice Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-17 Levi Lankford, Rupalatha Maddala, Monica M. Jablonski, P. Vasantha Rao
The etiology of elevated intraocular pressure (IOP), a major risk factor for glaucoma (optic nerve atrophy), is poorly understood despite continued efforts. Although the gene variant of (encoding α2δ1), a calcium voltage-gated channel auxiliary subunit, has been reported to be associated with primary open-angle glaucoma, and the pharmacological mitigation of α2δ1 activity by pregabalin lowers IOP,
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Allele-specific antisense oligonucleotides for the treatment of BEST1-related dominantly inherited retinal diseases: An in vitro model Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-16 Beren Karaosmanoglu, Gozde Imren, Eda Utine, Hande Taylan Sekeroglu, Ekim Z. Taskiran
Retinal dystrophies are a common health problem worldwide that are currently incurable due to the inability of retinal cells to regenerate. Inherited retinal diseases (IRDs) are a diverse group of disorders characterized by progressive vision loss caused by photoreceptor cell dysfunction. The eye has always been an attractive organ for the development of novel therapies due to its independent access
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Oxymatrine mitigates Aspergillus fumigatus keratitis by suppressing fungal activity and restricting pyroptosis Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-15 Weichen Liu, Xue Tian, Lingwen Gu, Bing Yu, Ziyi Wang, Menghui Chi, Jing Lin, Qian Wang, Guibo Liu, Guiqiu Zhao
Fungal keratitis (FK) is a refractory keratitis caused by excessive inflammation and fungal damage. Excessive inflammation can lead to tissue damage and corneal opacity, resulting in a poor prognosis for FK. Oxymatrine (OMT) is a natural alkaloid, which has rich pharmacological effects, such as antioxidant and anti-inflammation. However, its antifungal activity and the mechanism of action in FK have
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Gut microbiota compositional profile in patients with posner-schlossman syndrome Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-13 Ning Wang, Chengyang Sun, Yahan Ju, Lulu Huang, Yixin Liu, Mengyang Gu, Chenrui Xu, Minghan Wang, Yue Wu, Dandan Zhang, Li Xu, Wenyi Guo
The cause of Posner-Schlossman syndrome (PSS) remains unknown and its frequent recurrence may eventually lead to irreversible damage of the optic nerve. The influence of immune factors in the pathophysiology of PSS is gaining more and more interest. Increasing evidence suggests that gut dysbiosis plays vital roles in a variety of neurodegenerative and immune-related diseases. However, alterations of
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Verification of the gene and protein expression of the aquaglyceroporin AQP3 in the mammalian lens Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-12 Rosica S. Petrova, Nishanth Francis, Kevin L. Schey, Paul J. Donaldson
Transport of water is critical for maintaining the transparency of the avascular lens, and the lens is known to express at least five distinctly different water channels from the Aquaporin (AQP) family of proteins. In this study we report on the identification of a sixth lens AQP, AQP3 an aquaglyceroporin, which in addition to water also transports glycerol and HO. AQP3 was identified at the transcript
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CircRNA expression profiles and regulatory networks in the vitreous humor of people with high myopia Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-12 Liyue Zhang, Xin Yu, Nan Hong, Yutong Xia, Xuhong Zhang, Liyin Wang, Chen Xie, Feng Dong, Jianping Tong, Ye Shen
Myopia is a global health and economic issue. Circular RNAs (circRNAs) have been shown to play an important role in the pathogenesis of many ocular diseases. We first evaluated the circRNA profiles and possible roles in vitreous humor samples of individuals with high myopia by a competitive endogenous RNA (ceRNA) array. Vitreous humor samples were collected from 15 high myopic (5 for ceRNA array, and
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MiR-181a-5p may regulate cell proliferation and autophagy in myopia and the associated retinopathy Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-12 Bo Jiang, Nan Hong, Liyue Zhang, Baisheng Xu, Qin He, Xilin Qian, Feidi Li, Feng Dong
The mechanism of myopia and the associated retinopathy remains unclear, and dysregulated microRNAs (miRNAs) are implicated in this disease. In this research, we purposed to find out the regulatory function that miRNAs play in myopia and the associated retinopathy. We first performed miRNA microarray analysis in a lens-induced myopia mouse model and found that miR-9-5p, miR-96–5p, miR-182–5p, miR-183–5p
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Revisiting rabbit models for keratoconus: A long-term study on collagenase-induced disease progression Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-11 Sujithra Shankar, Minal Thacker, Abhishek Sahoo, Aniruth Reddy Aindla, Khyathi Ratna Padala, Jilu Jaffet, Kiran Kumar Bokara, Sayan Basu, Vivek Singh
Keratoconus (KC) is a degenerative disorder resulting from the degradation of the stromal collagen fibril network in the cornea, leading to its thinning and conical deformation. Various studies have established animal models of KC by using the collagenase type II enzyme to gain a better understanding of the pathogenesis, however, long-term monitoring or follow-up of the models have not been reported
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GSDMD mediated pyroptosis induced inflammation of Graves’ orbitopathy via the NF-κB/ AIM2/ Caspase-1 pathway Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-09 Xiaotong Sha, Huijing Ye, Xing Wang, Zhihui Xu, Anqi Sun, Wei Xiao, Te Zhang, Shenglan Yang, Huasheng Yang
Gasdermin D (GSDMD) is a key executor which triggers pyroptosis as well as an attractive checkpoint in various inflammatory and autoimmune diseases but it has yet to prove its function in Graves'orbitopathy (GO). Our aim was to investigate GSDMD levels in orbital connective tissue and serum of GO patients and then assess the association between serum levels and patients' clinical activity score (CAS)
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Comprehensive analysis of the miRNA-mRNA regulatory network involved in spontaneous recovery of an H2O2-induced zebrafish cataract model Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-09 Jiawei Luo, Mu Zhang, Yanhua Chen, Guowei Zhang, Tianqiu Zhou, Lihua Kang, Xiaoqing Chen, Huaijin Guan
To identify the hub miRNAs and mRNAs contributing to the spontaneous recovery of an HO-induced zebrafish cataract model. Zebrafishes were divided into three groups, i.e., Group A, which included normal control fish (day 0), and Groups B and C, where fish were injected with 2.5% hydrogen peroxide into the anterior chamber and reared for 14 and 30 days, respectively. Fish eyes were examined by stereomicroscope
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CircSTRBP contributes to H2O2-induced lens epithelium cell dysfunction through increasing NOX4 mRNA stability by recruiting IGF2BP1 Exp. Eye Res. (IF 3.4) Pub Date : 2024-02-09 Di Li, Xuanyi Che, Ningning Gao, Jing Li
Previous studies have shown that the development of age-related cataract (ARC) is involved in lens epithelium dysfunction, which is associated with abnormally expressed circular RNAs (circRNAs). The current work aims to probe the role of circSTRBP (hsa_circ_0088,427) in hydrogen peroxide (HO)-induced lens epitheliums. Lens epithelium tissues were harvested from ARC or normal subjects (n = 23). CircSTRBP