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Metabolic modeling of Halomonas campaniensis improves polyhydroxybutyrate production under nitrogen limitation Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-25 Carolina Deantas-Jahn, Sebastián N. Mendoza, Cuauhtemoc Licona-Cassani, Camila Orellana, Pedro A. Saa
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Sphingomonas lacusdianchii sp. nov., an attached bacterium inhibited by metabolites from its symbiotic cyanobacterium Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-25 Xin Wang, Yao Xiao, Yang Deng, Xue Sang, Qing-Lin Deng, Le Wang, Yi-Wen Yang, Bing-Huo Zhang, Yu-Qin Zhang
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Explainable AI for CHO cell culture media optimization and prediction of critical quality attribute Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-24 Neelesh Gangwar, Keerthiveena Balraj, Anurag S. Rathore
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Antibody-independent surface plasmon resonance assays for influenza vaccine quality control Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-24 Benjamin Serafin, Amine Kamen, Gregory de Crescenzo, Olivier Henry
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An overview of the two-component system GarR/GarS role on antibiotic production in Streptomyces coelicolor Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-24 Rodrigo Cruz-Bautista, Augusto Zelarayan-Agüero, Beatriz Ruiz-Villafán, Adelfo Escalante-Lozada, Romina Rodríguez-Sanoja, Sergio Sánchez
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Application of an alchemical free energy method for the prediction of thermostable DuraPETase variants Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-21 Sebastian Schreiber, David Gercke, Florian Lenz, Joachim Jose
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Improving the production of carbamoyltobramycin by an industrial Streptoalloteichus tenebrarius through metabolic engineering Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-21 Yun Feng, Yiqi Jiang, Xutong Chen, Li Zhu, Hailong Xue, Mianbin Wu, Lirong Yang, Haoran Yu, Jianping Lin
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Enzymatic and non-enzymatic removal of organic micropollutants with spent mushroom substrate of Agaricus bisporus Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-19 Brigit van Brenk, Fleur E.L. Kleijburg, Antoine J.B. Kemperman, Walter G.J. van der Meer, Han A.B. Wösten
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Alternative splicing analysis of lignocellulose-degrading enzyme genes and enzyme variants in Aspergillus niger Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-19 Yifan Xu, Feiyu Dong, Ruoxin Wang, Maria Ajmal, Xinyu Liu, Hui Lin, Hongge Chen
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Gn protein expressed in plants for diagnosis of severe fever with thrombocytopenia syndrome virus Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-19 Yu-Chih Chang, Hiroshi Shimoda, Min-chao Jiang, Yau-Heiu Hsu, Ken Maeda, Yumiko Yamada, Wei-Li Hsu
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Structural characteristics of gut microbiota in longevity from Changshou town, Hubei, China Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-15 Xu Ai, Yu Liu, Jinrong Shi, Xiongwei Xie, Linzi Li, Rui Duan, Yongling Lv, Kai Xiong, Yuanxin Miao, Yonglian Zhang
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Isolation and characterization of novel Staphylococcus aureus bacteriophage Hesat from dairy origin Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-15 Barbara Turchi, Claudia Campobasso, Arianna Nardinocchi, Jeroen Wagemans, Beatrice Torracca, Cédric Lood, Graziano Di Giuseppe, Paola Nieri, Fabrizio Bertelloni, Luca Turini, Valeria Ruffo, Rob Lavigne, Mariagrazia Di Luca
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Updated component analysis method for naturally occurring sophorolipids from Starmerella bombicola Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-12 Yosuke Kobayashi, Qiushi Li, Kazunori Ushimaru, Makoto Hirota, Tomotake Morita, Tokuma Fukuoka
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Pectate lyase genes from Radopholus similis and their application in pathotype identification Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-12 Sihua Yang, Shuai Yang, Qianying Li, Yang Lu, Xin Huang, Chun Chen, Chunling Xu, Hui Xie
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Strategies for the synthesis of the osmolyte glucosylglycerate and its precursor glycerate Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-12 Yentl Allaert, Arthur Leyder, Jorick Franceus, Tom Desmet
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Influence of combined abiotic/biotic factors on decay of P. aeruginosa and E. coli in Rhine River water Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-10 Sha Gao, Nora B. Sutton, Thomas V. Wagner, Huub H. M. Rijnaarts, Paul W. J. J. van der Wielen
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Manganese reductive dissolution coupled to Sb mobilization in contaminated shooting range soil Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-10 Lara Costa, Mathieu Martinez, Marcel Suleiman, Rolf Keiser, Moritz Lehmann, Markus Lenz
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Transcriptome analysis of Kluyveromyces marxianus under succinic acid stress and development of robust strains Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-09 Du-Wen Zeng, Yong-Qiang Yang, Qi Wang, Feng-Li Zhang, Mao-Dong Zhang, Sha Liao, Zhi-Qiang Liu, Ya-Chao Fan, Chen-Guang Liu, Lin Zhang, Xin-Qing Zhao
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Melanin depletion affects Aspergillus flavus conidial surface proteins, architecture, and virulence Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-09 Ondippili Rudhra, Hariharan Gnanam, Sivaramakrishnan Sivaperumal, VenkateshPrajna Namperumalsamy, Lalitha Prajna, Dharmalingam Kuppamuthu
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Pulchinenoside B4 ameliorates oral ulcers in rats by modulating gut microbiota and metabolites Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-09 Dewei Luo, Li Yan, Zhujun Wang, Xiaofan Ji, Na Pei, Jing Jia, Yingying Luo, Hui Ouyang, Shilin Yang, Yulin Feng
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Enhancing Escherichia coli abiotic stress resistance through ornithine lipid formation Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-08 Leidy Patricia Bedoya-Pérez, Alejandro Aguilar-Vera, Mishael Sánchez-Pérez, José Utrilla, Christian Sohlenkamp
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Epiberberine: a potential rumen microbial urease inhibitor to reduce ammonia release screened by targeting UreG Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-08 Xiaoyin Zhang, Zhanbo Xiong, Yue He, Nan Zheng, Shengguo Zhao, Jiaqi Wang
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A candidate competitive ELISA based on monoclonal antibody 3A8 for diagnosis of contagious bovine pleuropneumonia Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-08 Qi Wu, Zhixin Ma, Qiao Pan, Tong Liu, Yidan Zhang, Jiuqing Xin, Qingyuan Xu
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Associations between the rumen microbiota and carcass merit and meat quality in beef cattle Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-06 Devin B. Holman, Katherine E. Gzyl, Haley Scott, Nuria Prieto, Óscar López-Campos
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Cell density and extracellular matrix composition mitigate bacterial biofilm sensitivity to UV-C LED irradiation Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-05 Maritxu Labadie, Frédéric Marchal, Nofel Merbahi, Elisabeth Girbal-Neuhauser, Catherine Fontagné-Faucher, Claire-Emmanuelle Marcato-Romain
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IMPATIENT-qPCR: monitoring SELEX success during in vitro aptamer evolution Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-04 Ann-Kathrin Kissmann, Grigory Bolotnikov, Runliu Li, Franziska Müller, Hu Xing, Markus Krämer, Kay-E. Gottschalk, Jakob Andersson, Tanja Weil, Frank Rosenau
Abstract SELEX (Systematic Evolution of Ligands by Exponential enrichment) processes aim on the evolution of high-affinity aptamers as binding entities in diagnostics and biosensing. Aptamers can represent game-changers as constituents of diagnostic assays for the management of instantly occurring infectious diseases or other health threats. Without in-process quality control measures SELEX suffers
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Novel glycosidase from Paenibacillus lactis 154 hydrolyzing the 28-O-β-d-glucopyranosyl ester bond of oleanane-type saponins Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-04 Zongzhan Wu, Wenyu Dou, Xiaolin Yang, Tengfei Niu, Zhuzhen Han, Li Yang, Rufeng Wang, Zhengtao Wang
Abstract Oleanane-type ginsenosides are a class of compounds with remarkable pharmacological activities. However, the lack of effective preparation methods for specific rare ginsenosides has hindered the exploration of their pharmacological properties. In this study, a novel glycoside hydrolase PlGH3 was cloned from Paenibacillus lactis 154 and heterologous expressed in Escherichia coli. Sequence analysis
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Novel method for production and purification of untagged pneumococcal surface protein A from clade 1 Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-04 Tasson da Costa Rodrigues, Patricia Zorzete, Eliane Namie Miyaji, Viviane Maimoni Gonçalves
Abstract Streptococcus pneumoniae can cause diseases with high mortality and morbidity. The licensed vaccines are based on capsular polysaccharides and induce antibodies with low cross reactivity, leading to restricted coverage of serotypes. For surpassing this limitation, new pneumococcal vaccines are needed for induction of broader protection. One important candidate is the pneumococcal surface protein
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Enhancing the productivity and proliferation of CHO-K1 cells by oncoprotein YAP (Yes-associated protein) Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-04 Farnaz Roshanmehr, Shahriyar Abdoli, Zahra Bazi, Maryam Jari, Majid Shahbazi
Abstract CHO cells are extensively employed in biological drug industry to manufacture therapeutic proteins. Nevertheless, production of biopharmaceuticals faces obstacles such as limited growth and inadequate productivity. Employing host cell engineering techniques for CHO cells serves as a valuable approach to address the constraints encountered in biologics manufacturing. Despite advancements, most
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Quantum dot fluorescent microsphere-based immunochromatographic strip for detecting PRRSV antibodies Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-04 Rui Yang, Yi Ru, Huibao Wang, Rongzeng Hao, Yajun Li, Tao Zhang, Haixue Zheng, Yong Zhang, Xingxu Zhao
Abstract Porcine reproductive and respiratory syndrome (PRRS) is an immunosuppressive disease caused by the porcine reproductive and respiratory syndrome virus (PRRSV). Current vaccine prevention and treatment approaches for PRRS are not adequate, and commercial vaccines do not provide sufficient cross-immune protection. Therefore, establishing a precise, sensitive, simple, and rapid serological diagnostic
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A novel thermotolerant l-rhamnose isomerase variant for biocatalytic conversion of d-allulose to d-allose Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-02 Sweety Sharma, Satya Narayan Patel, Sudhir P. Singh
Abstract A novel l-rhamnose isomerase was identified and cloned from an extreme-temperature aquatic habitat metagenome. The deduced amino acid sequence homology suggested the possible source of this metagenomic sequence to be Chloroflexus islandicus. The gene expression was performed in a heterologous host, Escherichia coli, and the recombinant protein l-rhamnose isomerase (L-RIM) was extracted and
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Non-coding regulatory sRNAs from bacteria of the Burkholderia cepacia complex Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-02 Gonçalo R. Matos, Joana R. Feliciano, Jorge H. Leitão
Abstract Small non-coding RNAs (sRNAs) are key regulators of post-transcriptional gene expression in bacteria. Hundreds of sRNAs have been found using in silico genome analysis and experimentally based approaches in bacteria of the Burkholderia cepacia complex (Bcc). However, and despite the hundreds of sRNAs identified so far, the number of functionally characterized sRNAs from these bacteria remains
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Mutation breeding of high-stress resistant strains for succinic acid production from corn straw Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-04-01 Jing Wu, Yilian Li, Jinbao Yin, Chen Wang, Xuejin Qi, Yujie Zhou, Hongjuan Liu, Pengfei Wu, Jianan Zhang
Abstract The production of succinic acid from corn stover is a promising and sustainable route; however, during the pretreatment stage, byproducts such as organic acids, furan-based compounds, and phenolic compounds generated from corn stover inhibit the microbial fermentation process. Selecting strains that are resistant to stress and utilizing nondetoxified corn stover hydrolysate as a feedstock
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Assimilation of phthalate esters in bacteria Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-27 Pei Qiao, Tongtong Ying, Mengjie Gu, Jiahong Zhu, Chengyu Mei, Tong Hu, Tengfei Liu, Haixia Wang, Weihong Zhong
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Fighting pathogenic yeasts with plant defensins and anti-fungal proteins from fungi Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-27
Abstract Fungal infections represent a significant health risk worldwide. Opportunistic infections caused by yeasts, particularly by Candida spp. and their virulent emerging isolates, have become a major threat to humans, with an increase in fatal cases of infections attributed to the lack of effective anti-yeast therapies and the emergence of fungal resistance to the currently applied drugs. In this
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Biochemical characterization of a multiple prenyltransferase from Tolypocladium inflatum Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-26 Haiyan Han, Shuang Peng, Qian Wang, Hongwei Wang, Pengchao Wang, Chang Li, Jianzhao Qi, Chengwei Liu
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Effects of process intensification on homogeneity of an IgG1:κ monoclonal antibody during perfusion culture Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-26 George Liang, Chikkathur N. Madhavarao, Caitlin Morris, Thomas O’Connor, Muhammad Ashraf, Seongkyu Yoon
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Polyhydroxyalkanoate involvement in stress-survival of two psychrophilic bacterial strains from the High Arctic Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-23
Abstract An ever-growing body of literature evidences the protective role of polyhydroxyalkanoates (PHAs) against a plethora of mostly physical stressors in prokaryotic cells. To date, most of the research done involved bacterial strains isolated from habitats not considered to be life-challenging or extremely impacted by abiotic environmental factors. Polar region microorganisms experience a multitude
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Quorum quenching effect of cyclodextrins on the pyocyanin and pyoverdine production of Pseudomonas aeruginosa Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-22 Ildikó Fekete-Kertész, Zsófia Berkl, Kata Buda, Éva Fenyvesi, Lajos Szente, Mónika Molnár
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Regulation of the PFK1 gene on the interspecies microbial competition behavior of Saccharomyces cerevisiae Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-22 Caijuan Zheng, Shuxin Hou, Yu Zhou, Changyuan Yu, Hao Li
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Thermophilic cyanobacteria—exciting, yet challenging biotechnological chassis Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-21 Faiz Rasul, Dawei You, Ying Jiang, Xiangjian Liu, Maurycy Daroch
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Photoheterotroph improved the growth and nutrient levels of Chlorella vulgaris and the related molecular mechanism Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-20
Abstract Microalgae are rich in fatty acids, proteins, and other nutrients, which have gained the general attention of researchers all over the world. For the development of Chlorella vulgaris in food and feed industry, this study was conducted to investigate the differences in C. vulgaris’ growth and nutritional components under different culture conditions (autotrophic, heterotrophic, photoheterotrophic)
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Rare earth contamination of edible vegetation: Ce, La, and summed REE in fungi Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-20 Jerzy Falandysz, Anna Kilanowicz, Alwyn R. Fernandes, Ji Zhang
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Versatile ferrous oxidation–xylenol orange assay for high-throughput screening of lipoxygenase activity Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-18
Abstract Lipoxygenases (LOXs) catalyze dioxygenation of polyunsaturated fatty acids (PUFAs) into fatty acid hydroperoxides (FAHPs), which can be further transformed into a number of value-added compounds. LOXs have garnered interest as biocatalysts for various industrial applications. Therefore, a high-throughput LOX activity assay is essential to evaluate their performance under different conditions
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Unveiling the repressive mechanism of a PPS-like regulator (PspR) in polyhydroxyalkanoates biosynthesis network Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-18 Junyu Chen, Yinglu Cui, Shengjie Zhang, Bian Wu, Jing Han, Hua Xiang
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Characterization of the ADP-β-d-manno-heptose biosynthetic enzymes from two pathogenic Vibrio strains Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-18 Zhaoxiang Shi, Yue Tang, Zhenyi Wang, Min Wang, Zijian Zhong, Jingming Jia, Yihua Chen
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Cyanophycin modifications for applications in tissue scaffolding Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-15 Natalia Kwiatos, Deniz Atila, Michał Puchalski, Vignesh Kumaravel, Alexander Steinbüchel
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Adaptive responses of erythritol-producing Yarrowia lipolytica to thermal stress after evolution Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-15 Kai Xia, Yuqing Chen, Fangmei Liu, Xuequn Zhao, Ruyi Sha, Jun Huang
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Roles of pH and phosphate in rare earth element biosorption with living acidophilic microalgae Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-14
Abstract The increasing demand for rare earth elements (REEs) has spurred interest in the development of recovery methods from aqueous waste streams. Acidophilic microalgae have gained attention for REE biosorption as they can withstand high concentrations of transition metals and do not require added organic carbon to grow, potentially allowing simultaneous sorption and self-replication of the sorbent
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Dynamics and regulatory role of circRNAs in Asian honey bee larvae following fungal infection Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-13
Abstract Non-coding RNA (ncRNA) plays a vital part in the regulation of immune responses, growth, and development in plants and animals. Here, the identification, characteristic analysis, and molecular verification of circRNAs in Apis cerana cerana worker larval guts were conducted, followed by in-depth investigation of the expression pattern of larval circRNAs during Ascosphaera apis infection and
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Pharmacokinetics and pharmacodynamics of antibacterial peptide NZX in Staphylococcus aureus mastitis mouse model Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-13
Abstract Staphylococcus aureus is associated with dairy mastitis, which causes serious economic losses to dairy farming industry. Antibacterial peptide NZX showed good antibacterial activity against S. aureus. This study aimed to evaluate pharmacokinetics and pharmacodynamics of NZX against S. aureus-induced mouse mastitis. NZX exhibited potent in vitro antibacterial activity against the test S. aureus
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Computational aptamer design for spike glycoprotein (S) (SARS CoV-2) detection with an electrochemical aptasensor Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-12 Alessia Cossettini, Laura Pasquardini, Antonello Romani, Aldo Feriani, Debora Pinamonti, Marisa Manzano
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Exploring Rhodospirillum rubrum response to high doses of carbon monoxide under light and dark conditions Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-11 Manuel S. Godoy, Irene Verdú, Santiago R. de Miguel, José D. Jiménez, M. Auxiliadora Prieto
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Effects of inoculation and dry matter content on microbiome dynamics and metabolome profiling of sorghum silage Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-08 Zohreh Akhavan Kharazian, Dongmei Xu, Rina Su, Xusheng Guo
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Expression, purification, and characterization of transmembrane protein homogentisate solanesyltransferase Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-07 Han Xiao, Long-Can Mei, Hong-Yan Lin, Zhao Chen, Xin-He Yu, Jun Yang, Qiong Tong, Guang-Fu Yang
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The serine/threonine protein kinase MpSTE1 directly governs hyphal branching in Monascus spp. Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-06
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Identifying environmental factors affecting the microbial community composition on outdoor structural timber Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-06
Abstract Timber wood is a building material with many positive properties. However, its susceptibility to microbial degradation is a major challenge for outdoor usage. Although many wood-degrading fungal species are known, knowledge on their prevalence and diversity causing damage to exterior structural timber is still limited. Here, we sampled 46 decaying pieces of wood from outdoor constructions
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Black yeasts in hypersaline conditions Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-05 Cene Gostinčar, Nina Gunde-Cimerman
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Synergistic corrosion effects of magnetite and microorganisms: microbial community dependency Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-05 Maria A. Diaz-Mateus, Laura L. Machuca, Hanan Farhat, Silvia J. Salgar-Chaparro
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Mutational analysis in Corynebacterium stationis MFS transporters for improving nucleotide bioproduction Appl. Microbiol. Biotechnol. (IF 5.0) Pub Date : 2024-03-04 Keita Kinose, Keiko Shinoda, Tomoyuki Konishi, Hisashi Kawasaki