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Developing the Common Marmoset as a Translational Geroscience Model to Study the Microbiome and Healthy Aging Microorganisms (IF 4.5) Pub Date : 2024-04-25 Kelly R. Reveles, Alexana J. Hickmott, Kelsey A. Strey, Aaryn C. Mustoe, Juan Pablo Arroyo, Michael L. Power, Benjamin J. Ridenhour, Katherine R. Amato, Corinna N. Ross
Emerging data support associations between the depletion of the healthy gut microbiome and aging-related physiological decline and disease. In humans, fecal microbiota transplantation (FMT) has been used successfully to restore gut microbiome structure and function and to treat C. difficile infections, but its application to healthy aging has been scarcely investigated. The marmoset is an excellent
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Fodinisporobacter ferrooxydans gen. nov., sp. nov.—A Spore-Forming Ferrous-Oxidizing Bacterium Isolated from a Polymetallic Mine Microorganisms (IF 4.5) Pub Date : 2024-04-25 Zhen Jiang, Xiutong Li, Zonglin Liang, Zebao Tan, Nan Zhou, Ying Liu, Zhenghua Liu, Huaqun Yin, Kun Luo, Supawadee Ingsriswang, Shuangjiang Liu, Chengying Jiang
A novel acidophilic, aerobic bacterium strain, MYW30-H2T, was isolated from a heap of polymetallic mine. Cells of strain MYW30-H2T were Gram-stain-positive, endospore-forming, motile, and rod-shaped. Strain MYW30-H2T grew at a temperature range of 30–45 °C (optimum 40 °C) and a pH range of 3.5–6.0 (optimum 4.0) in the presence of 0–0.5% (w/v) NaCl. Strain MYW30-H2T could grow heterotrophically on yeast
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The Impact of Artificial Restoration of Alpine Grasslands in the Qilian Mountains on Vegetation, Soil Bacteria, and Soil Fungal Community Diversity Microorganisms (IF 4.5) Pub Date : 2024-04-25 Xiaomei Yang, Qi Feng, Meng Zhu, Jutao Zhang, Linshan Yang, Ruolin Li
To understand how the soil microbial community structure responds to vegetation restoration in alpine mining areas, this study specifically examines the grassland ecosystem in the Qianmalong mining area of the Qilian Mountains after five years of artificial restoration. High-throughput sequencing methods were employed to analyze soil bacteria and fungi microbial characteristics in diverse grassland
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Trichinella spiralis Infecting Wild Boars in West, Southwest, and Northwest of Romania: Evidence of an Underrated Risk Microorganisms (IF 4.5) Pub Date : 2024-04-25 Ana-Maria Marin, Tudor Rareș Olariu, Dan-Cornel Popovici, Gianluca Marucci, Sorin Morariu, Daian Popa, Narcisa Mederle
The species of the genus Trichinella are etiological agents distributed all over the world and are able to infect mammals, birds, and reptiles. Trichinella spiralis is the species most adapted to domestic and wild pigs and is also the most important etiological agent of trichinellosis. The wild boar (Sus scrofa) is a nocturnal omnivorous mammal belonging to the Suidae family. S. scrofa has a great
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From Investigating a Case of Cellulitis to Exploring Nosocomial Infection Control of ST1 Legionella pneumophila Using Genomic Approaches Microorganisms (IF 4.5) Pub Date : 2024-04-25 Charlotte Michel, Fedoua Echahidi, Sammy Place, Lorenzo Filippin, Vincent Colombie, Nicolas Yin, Delphine Martiny, Olivier Vandenberg, Denis Piérard, Marie Hallin
Legionella pneumophila can cause a large panel of symptoms besides the classic pneumonia presentation. Here we present a case of fatal nosocomial cellulitis in an immunocompromised patient followed, a year later, by a second case of Legionnaires’ disease in the same ward. While the first case was easily assumed as nosocomial based on the date of symptom onset, the second case required clear typing
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Temporal Dynamics of Fungal Communities in Alkali-Treated Round Bamboo Deterioration under Natural Weathering Microorganisms (IF 4.5) Pub Date : 2024-04-25 Shuaibo Han, Xiaojiao An, Xiaolong He, Xin Ren, John Sichone, Xinxing Wu, Yan Zhang, Hui Wang, Fangli Sun
Microbes naturally inhabit bamboo-based materials in outdoor environments, sequentially contributing to their deterioration. Fungi play a significant role in deterioration, especially in environments with abundant water and favorable temperatures. Alkali treatment is often employed in the pretreatment of round bamboo to change its natural elastic and aesthetic behaviors. However, little research has
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Interaction of Trypanosoma cruzi, Triatomines and the Microbiota of the Vectors—A Review Microorganisms (IF 4.5) Pub Date : 2024-04-25 Günter A. Schaub
This review summarizes the interactions between Trypanosoma cruzi, the etiologic agent of Chagas disease, its vectors, triatomines, and the diverse intestinal microbiota of triatomines, which includes mutualistic symbionts, and highlights open questions. T. cruzi strains show great biological heterogeneity in their development and their interactions. Triatomines differ from other important vectors
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Editorial: Impact of Special Issue “The Microbial Population of the Gastrointestinal Tract of Animals: Impacts on Host Physiology” Microorganisms (IF 4.5) Pub Date : 2024-04-25 Jeferson M. Lourenco, Todd R. Callaway
In recent years, there has been an exponential increase in the number of papers that have investigated the microbiome of animals and humans [...]
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Multi-Omics of Campylobacter jejuni Growth in Chicken Exudate Reveals Molecular Remodelling Associated with Altered Virulence and Survival Phenotypes Microorganisms (IF 4.5) Pub Date : 2024-04-25 Lok Man, Pamela X. Y. Soh, Tess E. McEnearney, Joel A. Cain, Ashleigh L. Dale, Stuart J. Cordwell
Campylobacter jejuni is the leading cause of foodborne human gastroenteritis in the developed world. Infections are largely acquired from poultry produced for human consumption and poor food handling is thus a major risk factor. Chicken exudate (CE) is a liquid produced from defrosted commercial chicken products that facilitates C. jejuni growth. We examined the response of C. jejuni to growth in CE
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Immune Response after Anti-SARS-CoV-2 mRNA Vaccination in Relation to Cellular Immunity, Vitamin D and Comorbidities in Hemodialysis Patients Microorganisms (IF 4.5) Pub Date : 2024-04-25 Egle Dalinkeviciene, Brigita Gradauskiene, Sandra Sakalauskaite, Kristina Petruliene, Ruta Vaiciuniene, Inga Skarupskiene, Daina Bastyte, Jolanta Sauseriene, Leonas Valius, Inga Arune Bumblyte, Edita Ziginskiene
In the global threat of SARS-CoV-2, individuals undergoing maintenance dialysis represent a vulnerable population with an increased risk of severe COVID-19 outcomes. Therefore, immunization against SARS-CoV-2 is an essential component of healthcare strategy for these patients. Existing data indicate that they tend to exhibit a reduced immune response to vaccines compared to the general population.
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Surfing the Waves of SARS-CoV-2: Analysis of Viral Genome Variants Using an NGS Survey in Verona, Italy Microorganisms (IF 4.5) Pub Date : 2024-04-24 Emil Tonon, Riccardo Cecchetto, Erica Diani, Nicoletta Medaina, Giona Turri, Anna Lagni, Virginia Lotti, Davide Gibellini
The availability of new technologies for deep sequencing, including next-generation sequencing (NGS), allows for the detection of viral genome variations. The epidemiological determination of SARS-CoV-2 viral genome changes during the pandemic waves displayed the genome evolution and subsequent onset of variants over time. These variants were often associated with a different impact on viral transmission
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Evaluation of the Increased Genetic Resolution and Utility for Source Tracking of a Recently Developed Method for Genotyping Cyclospora cayetanensis Microorganisms (IF 4.5) Pub Date : 2024-04-24 Susan R. Leonard, Mark K. Mammel, Sonia Almeria, Solomon T. Gebru, David K. Jacobson, Anna C. Peterson, Joel L. N. Barratt, Steven M. Musser
Cyclospora cayetanensis is a foodborne parasite that causes cyclosporiasis, an enteric illness in humans. Genotyping methods are used to genetically discriminate between specimens from cyclosporiasis cases and can complement source attribution investigations if the method is sufficiently sensitive for application to food items. A very sensitive targeted amplicon sequencing (TAS) assay for genotyping
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Evidence for Immunity against Tetanus, Diphtheria, and Pertussis through Natural Infection or Vaccination in Adult Solid Organ Transplant Recipients: A Systematic Review Microorganisms (IF 4.5) Pub Date : 2024-04-24 Emil Lenzing, Zitta Barrella Harboe, Søren Schwartz Sørensen, Allan Rasmussen, Susanne Dam Nielsen, Omid Rezahosseini
(1) Background: We aim to systematically review the current evidence on immunity against tetanus, diphtheria, and pertussis in adult solid organ transplantation (SOT) recipients, either through natural infection or vaccination. (2) Methods: This systematic review was conducted per PRISMA guidelines. We assessed the risk of bias using the Cochrane RoB 2 and ROBINS-I and summarized the findings narratively
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Prosthetic Joint Infections Caused by Mycobacterium tuberculosis Complex—An ESGIAI–ESGMYC Multicenter, Retrospective Study and Literature Review Microorganisms (IF 4.5) Pub Date : 2024-04-24 Alvaro Auñon, Llanos Salar-Vidal, Ignacio Mahillo-Fernandez, Francisco Almeida, Pedro Pereira, Jaime Lora-Tamayo, Tristan Ferry, Sarah Souèges, Aurélien Dinh, Rosa Escudero, Candela Menéndez Fernández-Miranda, Alicia Rico, Nicolo Rossi, Jaime Esteban
Purpose: While tuberculosis remains a significant global health concern, prosthetic joint infections (PJIs) caused by members of the Mycobacterium tuberculosis complex are exceptionally rare. Our objective is to perform a retrospective search of new cases of this disease and analyze all cases available in the literature of tuberculous PJIs, aiming to detect factors that may influence patient outcomes
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Type I Cystatin Derived from Cysticercus pisiformis—Stefins, Suppresses LPS-Mediated Inflammatory Response in RAW264.7 Cells Microorganisms (IF 4.5) Pub Date : 2024-04-24 Qianqian Yang, Jia Li, Lilan Zhang, Ningning Zhao, Xiaolin Sun, Zexiang Wang
Cysticercus pisiformis is a kind of tapeworm larvae of Taenia pisiformis, which parasitizes the liver envelope, omentum, mesentery, and rectum of rodents such as rabbits. Cysteine protease inhibitors derived from helminth were immunoregulatory molecules of intermediate hosts and had an immunomodulatory function that regulates the production of inflammatory factors. Thus, in the present research, the
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Advances in Gut Microbiota-Targeted Therapeutics for Metabolic Syndrome Microorganisms (IF 4.5) Pub Date : 2024-04-24 Yu Gao, Wujuan Li, Xiaoyu Huang, Yuhong Lyu, Changwu Yue
Previous investigations have illuminated the significant association between the gut microbiome and a broad spectrum of health conditions, including obesity, diabetes, cardiovascular diseases, and psychiatric disorders. Evidence from certain studies suggests that dysbiosis of the gut microbiota may play a role in the etiology of obesity and diabetes. Moreover, it is acknowledged that dietary habits
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Chemical Characterization and Effect of a Lactobacilli-Postbiotic on Streptococcus mutans Biofilm In Vitro Microorganisms (IF 4.5) Pub Date : 2024-04-23 Guilherme Bandeira Santana, Patrick Veras Quelemes, Enedina Rodrigues da Silva Neta, Sidney Gonçalo de Lima, Gláuber Campos Vale
Postbiotic is the term used to define the soluble factors, metabolic products, or byproducts released by live probiotic bacteria or after its lysis. The objective of this study was to carry out the chemical characterization of the postbiotic of Lacticaseibacillus rhamnosus LR-32 and to evaluate its in vitro effect on the development of the Streptococcus mutans biofilm. After the cultivation of the
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Tackling the Antimicrobial Resistance “Pandemic” with Machine Learning Tools: A Summary of Available Evidence Microorganisms (IF 4.5) Pub Date : 2024-04-23 Doris Rusic, Marko Kumric, Ana Seselja Perisin, Dario Leskur, Josipa Bukic, Darko Modun, Marino Vilovic, Josip Vrdoljak, Dinko Martinovic, Marko Grahovac, Josko Bozic
Antimicrobial resistance is recognised as one of the top threats healthcare is bound to face in the future. There have been various attempts to preserve the efficacy of existing antimicrobials, develop new and efficient antimicrobials, manage infections with multi-drug resistant strains, and improve patient outcomes, resulting in a growing mass of routinely available data, including electronic health
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Genomic Characterization and Molecular Detection of Rehmannia Allexivirus Virus, a Novel Allexivirus Infecting Rehmannia glutinosa Microorganisms (IF 4.5) Pub Date : 2024-04-23 Yanhong Qin, Shuhao Lu, Yi Wen, Shaojian Li, Suxia Gao, Yuxia Liu, Xuemeng Li, Jin Yang, Fengli Wang, Fei Wang, Chuantao Lu
Rehmannia glutinosa is one of the most important medicinal plants in China and is affected by viral diseases. In this study, a new virus tentatively named Rehmannia Allexivirus virus (ReAV) was identified through high-throughput sequencing, reverse-transcription polymerase chain reaction (RT-PCR), and Sanger sequencing. The complete genome length was 7297 nt and it contained five open reading frames
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Comparative Genomics Reveals Genetic Diversity and Variation in Metabolic Traits in Fructilactobacillus sanfranciscensis Strains Microorganisms (IF 4.5) Pub Date : 2024-04-23 Xiaxia He, Yujuan Yu, Rober Kemperman, Luciana Jimenez, Faizan Ahmed Sadiq, Guohua Zhang
Fructilactobacillus sanfranciscensis is a significant and dominant bacterial species of sourdough microbiota from ecological and functional perspectives. Despite the remarkable prevalence of different strains of this species in sourdoughs worldwide, the drivers behind the genetic diversity of this species needed to be clarified. In this research, 14 F. sanfranciscensis strains were isolated from sourdough
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Lactobacillus acidophilus LA-5 Ameliorates Inflammation and Alveolar Bone Loss Promoted by A. actinomycetemcomitans and S. gordonii in Mice and Impacts Oral and Gut Microbiomes Microorganisms (IF 4.5) Pub Date : 2024-04-22 Manuela R. Bueno, Fernando H. Martins, Catarina M. Rocha, Dione Kawamoto, Karin H. Ishikawa, Ellen S. Ando-Suguimoto, Aline R. Carlucci, Leticia S. Arroteia, Renato V. Casarin, Marcia P. A. Mayer
The benefits of probiotics on dysbiotic microbiomes and inflammation are dependent on the tested strain, host factors, and the resident microbiome. There is limited knowledge on the effects of probiotics in A. actinomycetemcomitans-associated periodontitis. Thus, Lactobacillus acidophilus LA5 (LA5) was orally inoculated for 30 days in C57Bl/6 mice infected with A. actinomycetemcomitans JP2 (Aa) and
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Targeted Integration of siRNA against Porcine Cytomegalovirus (PCMV) Enhances the Resistance of Porcine Cells to PCMV Microorganisms (IF 4.5) Pub Date : 2024-04-22 Hongzhen Mao, Jinyang Li, Mengyu Gao, Xinmei Liu, Haohan Zhang, Yijia Zhuang, Tianyi He, Wei Zuo, Lang Bai, Ji Bao
In the world’s first pig-to-human cardiac cytomegalovirus (PCMV), xenotransplant and elevated levels of porcine key factors contributing to patient mortality were considered. This has renewed attention on PCMV, a virus widely prevalent in pigs. Currently, there are no effective drugs or vaccines targeting PCMV, and its high detection difficulty poses challenges for prevention and control research.
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Serological Evidence of Crimean–Congo Haemorrhagic Fever in Livestock in the Omaheke Region of Namibia Microorganisms (IF 4.5) Pub Date : 2024-04-22 Alaster Samkange, Pricilla Mbiri, Ophelia Chuma Matomola, Georgina Zaire, Anna Homateni, Elifas Junias, Israel Kaatura, Siegfried Khaiseb, Simson Ekandjo, Johannes Shoopala, Magrecia Hausiku, Albertina Shilongo, Mushabati Linus Mujiwa, Klaas Dietze, Frank Busch, Christian Winter, Carolina Matos, Sabrina Weiss, Simbarashe Chitanga
This research examined the positivity ratio of Crimean–Congo haemorrhagic fever (CCHF) antibodies in cattle and sheep within Namibia’s Omaheke region after a human disease outbreak in the same geographical area. A total of 200 samples (100 cattle and 100 sheep) were randomly collected from animals brought to two regional auction sites, and then tested using the ID Screen® CCHF Double Antigen Multi-Species
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Cell-Free Culture Supernatant of Lactobacillus acidophilus AG01 and Bifidobacterium animalis subsp. lactis AG02 Reduces the Pathogenicity of NetB-Positive Clostridium perfringens in a Chicken Intestinal Epithelial Cell Line Microorganisms (IF 4.5) Pub Date : 2024-04-22 Darshana Kadekar, Andreea Cornelia Udrea, Steffen Yde Bak, Niels Christensen, Kirsty Gibbs, Chong Shen, Marion Bernardeau
The worldwide reduction in the use of antibiotics in animal feed is fueling the need for alternatives for the prevention and control of poultry intestinal diseases such as necrotic enteritis (NE), which is caused by Clostridium perfringens. This is the first report on the use of an intestinal epithelial chicken cell line (CHIC-8E11) to study the pathogenic traits of C. perfringens and to investigate
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Investigation of Effects of Novel Bifidobacterium longum ssp. longum on Gastrointestinal Microbiota and Blood Serum Parameters in a Conventional Mouse Model Microorganisms (IF 4.5) Pub Date : 2024-04-22 Merle Rätsep, Kalle Kilk, Mihkel Zilmer, Sirje Kuusik, Liina Kuus, Mirjam Vallas, Oksana Gerulis, Jelena Štšepetova, Aivar Orav, Epp Songisepp
Representatives of the genus Bifidobacterium are widely used as probiotics to modulate the gut microbiome and alleviate various health conditions. The action mechanisms of probiotics rely on their direct effect on the gut microbiota and the local and systemic effect of its metabolites. The main purpose of this animal experiment was to assess the biosafety of the Bifidobacterium longum strain BIOCC1719
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A Comparative Transcriptome Analysis Unveils the Mechanisms of Response in Feather Degradation by Pseudomonas aeruginosa Gxun-7 Microorganisms (IF 4.5) Pub Date : 2024-04-22 Chaodong Song, Rui Liu, Doudou Yin, Chenjie Xie, Ying Liang, Dengfeng Yang, Mingguo Jiang, Hongyan Zhang, Naikun Shen
Microbial degradation of feathers offers potential for bioremediation, yet the microbial response mechanisms warrant additional investigation. In prior work, Pseudomonas aeruginosa Gxun-7, which demonstrated robust degradation of feathers at elevated concentrations, was isolated. However, the molecular mechanism of this degradation remains only partially understood. To investigate this, we used RNA
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Acute Hepatitis of Unknown Origin in Children: Analysis of 17 Cases Admitted to the Bambino Gesù Children’s Hospital in Rome Microorganisms (IF 4.5) Pub Date : 2024-04-19 Velia Chiara Di Maio, Leonarda Gentile, Rossana Scutari, Luna Colagrossi, Luana Coltella, Stefania Ranno, Giulia Linardos, Daniela Liccardo, Maria Sole Basso, Andrea Pietrobattista, Simona Landi, Lorena Forqué, Marta Ciofi Degli Atti, Lara Ricotta, Andrea Onetti Muda, Giuseppe Maggiore, Massimiliano Raponi, Carlo Federico Perno, Cristina Russo
This study described 17 cases of children admitted to the Bambino Gesù Children’s Hospital with acute hepatitis of unknown origin between mid-April and November 2022. Following the World Health Organization’s working case definition of probable cases, 17 children, with a median age of 2.1 years (interquartile range: 1.0–7.1), presenting with acute hepatitis non-AE, with serum transaminase >500 IU/L
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Priestia megaterium ASC-1 Isolated from Pickled Cabbage Ameliorates Hyperuricemia by Degrading Uric Acid in Rats Microorganisms (IF 4.5) Pub Date : 2024-04-20 Wenjuan Zhu, Siyuan Bi, Zhijia Fang, Lukman Iddrisu, Qi Deng, Lijun Sun, Ravi Gooneratne
Pickled cabbage, a traditional fermented food rich in functional microorganisms, can effectively control hyperuricemia and gout. In this study, a Priestia megaterium ASC-1 strain with strong uric acid (UA) degradation ability was isolated from pickled cabbage. After oral administration for 15 days, ASC-1 was stably colonized in the rats in this study. ASC-1 significantly reduced UA levels (67.24%)
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Perspectives of FTIR as Promising Tool for Pathogen Diagnosis, Sanitary and Welfare Monitoring in Animal Experimentation Models: A Review Based on Pertinent Literature Microorganisms (IF 4.5) Pub Date : 2024-04-20 Matheus Morais Neves, Renan Faria Guerra, Isabela Lima Lemos, Thomas Santos Arrais, Marco Guevara-Vega, Flávia Batista Ferreira, Rafael Borges Rosa, Mylla Spirandelli Vieira, Belchiolina Beatriz Fonseca, Robinson Sabino da Silva, Murilo Vieira da Silva
Currently, there is a wide application in the literature of the use of the Fourier Transform Infrared Spectroscopy (FTIR) technique. This basic tool has also proven to be efficient for detecting molecules associated with hosts and pathogens in infections, as well as other molecules present in humans and animals’ biological samples. However, there is a crisis in science data reproducibility. This crisis
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Unveiling Antibacterial Potential and Physiological Characteristics of Thermophilic Bacteria Isolated from a Hot Spring in Iran Microorganisms (IF 4.5) Pub Date : 2024-04-20 Zeinab Rafiee, Maryam Jalili Tabaii, Maryam Moradi, Sharareh Harirchi
The increasing worldwide demand for antimicrobial agents has significantly contributed to the alarming rise of antimicrobial resistance, posing a grave threat to human life. Consequently, there is a pressing need to explore uncharted environments, seeking out novel antimicrobial compounds that display exceptionally efficient capabilities. Hot springs harbor microorganisms possessing remarkable properties
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Growth and Cell Size of Microalga Auxenochlorella protothecoides AS-1 under Different Trophic Modes Microorganisms (IF 4.5) Pub Date : 2024-04-20 Haoyu Chen, Ana Sosa, Feng Chen
Certain microalgal species can grow with different trophic strategies depending on the availability of nutrient resources. They can use the energy from light or an organic substrate, or both, and can therefore be called autotrophs, heterotrophs, or mixotrophs. We recently isolated a microalgal strain from the microplastic biofilm, which was identified as Auxenochlorella protothecoides, AS-1. Strain
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Comparative Analysis of Bisexual and Parthenogenetic Populations in Haemaphysalis Longicornis Microorganisms (IF 4.5) Pub Date : 2024-04-19 Chaoyue Zhao, Guonan Cai, Xing Zhang, Xinyu Liu, Pengfei Wang, Aihua Zheng
Haemaphysalis longicornis, a three-host tick with a wide host range, is widely distributed in different countries and regions. It stands out among ticks due to its unique feature of having both parthenogenetic and bisexual populations. Despite their morphological resemblance, the characteristics of the parthenogenetic population have been overlooked. In this comprehensive study, we systematically compared
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The Changes in Fecal Bacterial Communities in Goats Offered Rumen-Protected Fat Microorganisms (IF 4.5) Pub Date : 2024-04-19 Hu Liu, Weishi Peng, Kaiyu Mao, Yuanting Yang, Qun Wu, Ke Wang, Meng Zeng, Xiaotao Han, Jiancheng Han, Hanlin Zhou
Leizhou goats are famous for their delicious meat but have inferior growth performance. There is little information on rumen-protected fat (RPF) from the Leizhou goat. Hence, we observed the effects of RPF on growth, fecal short-chain fatty acids, and bacteria community with respect to Leizhou goats. Twelve goats (13.34 ± 0.024 kg) were selected and assigned randomly to one of two treatments: (1) a
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Whole-Genome Sequencing and Phenotypic Analysis of Streptococcus equi subsp. zooepidemicus Sequence Type 147 Isolated from China Microorganisms (IF 4.5) Pub Date : 2024-04-19 Yan Su, Zehua Zhang, Li Wang, Baojiang Zhang, Lingling Su
Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is one of the important zoonotic and opportunistic pathogens. In recent years, there has been growing evidence that supports the potential role of S. zooepidemicus in severe diseases in horses and other animals, including humans. Furthermore, the clinical isolation and drug resistance rates of S. zooepidemicus have been increasing yearly, leading
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Evaluation of the Effects of Food Safety Training on the Microbiological Load Present in Equipment, Surfaces, Utensils, and Food Manipulator’s Hands in Restaurants Microorganisms (IF 4.5) Pub Date : 2024-04-19 Miguel Castro, Kamila Soares, Carlos Ribeiro, Alexandra Esteves
Training food handlers is essential to ensure food safety. However, the efficacy of training programs relying solely on theoretical information remains uncertain and often fails to induce significant changes in inadequate food practices. Training programs in good hygiene and food safety practices that integrate theoretical and practical approaches have emerged as a vital tool, enabling food handlers
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Neutrophils versus Protozoan Parasites: Plasmodium, Trichomonas, Leishmania, Trypanosoma, and Entameoba Microorganisms (IF 4.5) Pub Date : 2024-04-19 Eileen Uribe-Querol, Carlos Rosales
Neutrophils are the most abundant polymorphonuclear granular leukocytes in human blood and are an essential part of the innate immune system. Neutrophils are efficient cells that eliminate pathogenic bacteria and fungi, but their role in dealing with protozoan parasitic infections remains controversial. At sites of protozoan parasite infections, a large number of infiltrating neutrophils is observed
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Association of Acidotolerant Cyanobacteria to Microbial Mats below pH 1 in Acidic Mineral Precipitates in Río Tinto River in Spain Microorganisms (IF 4.5) Pub Date : 2024-04-19 Felipe Gómez, Nuria Rodríguez, José Antonio Rodríguez-Manfredi, Cristina Escudero, Ignacio Carrasco-Ropero, José M. Martínez, Marco Ferrari, Simone De Angelis, Alessandro Frigeri, Maite Fernández-Sampedro, Ricardo Amils
This report describes acidic microbial mats containing cyanobacteria that are strongly associated to precipitated minerals in the source area of Río Tinto. Río Tinto (Huelva, Southwestern Spain) is an extreme acidic environment where iron and sulfur cycles play a fundamental role in sustaining the extremely low pH and the high concentration of heavy metals, while maintaining a high level of microbial
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Effect of Stool Sampling on a Routine Clinical Method for the Quantification of Six Short Chain Fatty Acids in Stool Using Gas Chromatography–Mass Spectrometry Microorganisms (IF 4.5) Pub Date : 2024-04-19 Tarek Mahdi, Aurore Desmons, Pranvera Krasniqi, Jean-Marc Lacorte, Nathalie Kapel, Antonin Lamazière, Salma Fourati, Thibaut Eguether
Short chain fatty acids (SCFAs) are primarily produced in the caecum and proximal colon via the bacterial fermentation of undigested carbohydrates that have avoided digestion in the small intestine. Increasing evidence supports the critical role that SCFAs play in health and homeostasis. Microbial SCFAs, namely butyric acid, serve as a principal energy source for colonocytes, and their production is
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Impact of Multidrug-Resistant Organisms on Severe Acquired Brain Injury Rehabilitation: An Observational Study Microorganisms (IF 4.5) Pub Date : 2024-04-19 Giovanna Barbara Castellani, Elisa Maietti, Valentina Colombo, Stefano Clemente, Ivo Cassani, Paola Rucci
Healthcare-associated infections (HAIa) and antimicrobial resistance are expected to be the next threat to human health and are most frequent in people with severe acquired brain injury (SABI), who can be more easily colonized by multidrug-resistant organisms (MDROs). The study’s aim is to investigate the impact of MDRO colonizations and infections on SABI rehabilitation outcomes. This retrospective
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Evaluation of Probiotic Properties and Safety of Lactobacillus helveticus LH10 Derived from Vinegar through Comprehensive Analysis of Genotype and Phenotype Microorganisms (IF 4.5) Pub Date : 2024-04-19 Yang Du, Jingru Xu, Jinquan Li, Renwei Wu
The probiotic potential of Lactobacillus helveticus LH10, derived from vinegar Pei, a brewing mixture, was assessed through genotype and phenotype analyses. The assembled genome was comprised of 1,810,276 bp and predicted a total of 2044 coding sequences (CDSs). Based on the whole genome sequence analysis, two bacteriocin gene clusters were identified, while no pathogenic genes were detected. In in
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Women Skin Microbiota Modifications during Pregnancy Microorganisms (IF 4.5) Pub Date : 2024-04-17 Giulia Radocchia, Francesca Brunetti, Massimiliano Marazzato, Valentina Totino, Bruna Neroni, Giulia Bonfiglio, Antonietta Lucia Conte, Fabrizio Pantanella, Paola Ciolli, Serena Schippa
Several studies have shown fluctuations in the maternal microbiota at various body sites (gut, oral cavity, and vagina). The skin microbiota plays an important role in our health, but studies on the changes during pregnancy are limited. Quantitative and qualitative variations in the skin microbiota in pregnant woman could indeed play important roles in modifying the immune and inflammatory responses
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Genomic Characterization of Listeria monocytogenes and Other Listeria Species Isolated from Sea Turtles Microorganisms (IF 4.5) Pub Date : 2024-04-18 Ludovica Di Renzo, Maria Elisabetta De Angelis, Marina Torresi, Giulia Mariani, Federica Pizzurro, Luana Fiorella Mincarelli, Emanuele Esposito, Maria Oliviero, Doriana Iaccarino, Fabio Di Nocera, Gianluigi Paduano, Giuseppe Lucifora, Cesare Cammà, Nicola Ferri, Francesco Pomilio
Listeria monocytogenes is a ubiquitous pathogen found both in the environment and food. It can cause listeriosis in a wide range of animals as well as in humans. Investigations on presence, spread and virulence are still limited to terrestrial and human environments. Embracing the One Health Approach, investigating the presence and spread of L. monocytogenes in marine ecosystems and among wildlife
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Non-O1/Non-O139 Vibrio cholerae—An Underestimated Foodborne Pathogen? An Overview of Its Virulence Genes and Regulatory Systems Involved in Pathogenesis Microorganisms (IF 4.5) Pub Date : 2024-04-18 Quantao Zhang, Thomas Alter, Susanne Fleischmann
In recent years, the number of foodborne infections with non-O1 and non-O139 Vibrio cholerae (NOVC) has increased worldwide. These have ranged from sporadic infection cases to localized outbreaks. The majority of case reports describe self-limiting gastroenteritis. However, severe gastroenteritis and even cholera-like symptoms have also been described. All reported diarrheal cases can be traced back
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Epidemiological and Clinical Aspects of Cutaneous and Mucosal Leishmaniases in Portugal: Retrospective Analysis of Cases Diagnosed in Public Hospitals and Reported in the Literature between 2010 and 2020 Microorganisms (IF 4.5) Pub Date : 2024-04-18 Rafael Rocha, Cláudia Conceição, Luzia Gonçalves, Ana Cláudia Carvalho, André Maia, André Martins, António Carujo, António Maio, Catarina Forra, Catarina Melita, Daniela Couto, Diana Fernandes, Dulce Pereira, Ema Leal, Helena Sarmento, Inês Sousa, Jean-Pierre Gonçalves, Joana Marinho, Joana Vasconcelos, João Cunha, João Rodrigues, José Miguel Silva, Lídia Caley, Luís Malheiro, Luís Santos, Margarida
Leishmania infantum, a zoonotic vector-born parasite, is endemic in the Mediterranean region, presenting mostly as visceral (VL), but also as cutaneous (CL) and mucosal leishmaniasis (ML). This study aimed to describe the epidemiological and clinical aspects of the CL and ML cases diagnosed in mainland Portugal between 2010 and 2020. Collaboration was requested from every hospital of the Portuguese
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Ligand-Free Silver Nanoparticles: An Innovative Strategy against Viruses and Bacteria Microorganisms (IF 4.5) Pub Date : 2024-04-18 Maria Vittoria Morone, Annalisa Chianese, Federica Dell’Annunziata, Veronica Folliero, Erwin Pavel Lamparelli, Giovanna Della Porta, Carla Zannella, Anna De Filippis, Gianluigi Franci, Massimiliano Galdiero, Antonio Morone
The spread of antibiotic-resistant bacteria and the rise of emerging and re-emerging viruses in recent years constitute significant public health problems. Therefore, it is necessary to develop new antimicrobial strategies to overcome these challenges. Herein, we describe an innovative method to synthesize ligand-free silver nanoparticles by Pulsed Laser Ablation in Liquid (PLAL-AgNPs). Thus produced
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Sex- and Gender-Based Analysis on Norepinephrine Use in Septic Shock: Why Is It Still a Male World? Microorganisms (IF 4.5) Pub Date : 2024-04-18 Benedetta Perna, Valeria Raparelli, Federica Tordo Caprioli, Oana Teodora Blanaru, Cecilia Malacarne, Cecilia Crosetti, Andrea Portoraro, Alex Zanotto, Francesco Maria Strocchi, Alessandro Rapino, Anna Costanzini, Martina Maritati, Roberto Lazzari, Michele Domenico Spampinato, Carlo Contini, Roberto De Giorgio, Matteo Guarino
Sex and gender are fundamental health determinants and their role as modifiers of treatment response is increasingly recognized. Norepinephrine is a cornerstone of septic shock management and its use is based on the highest level of evidence compared to dopamine. The related 2021 Surviving Sepsis Campaign (SCC) recommendation is presumably applicable to both females and males; however, a sex- and gender-based
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Current Insights into the Maturation of Epstein–Barr Virus Particles Microorganisms (IF 4.5) Pub Date : 2024-04-17 Asuka Nanbo
The three subfamilies of herpesviruses (alphaherpesviruses, betaherpesviruses, and gammaherpesviruses) appear to share a unique mechanism for the maturation and egress of virions, mediated by several budding and fusion processes of various organelle membranes during replication, which prevents cellular membrane disruption. Newly synthesized viral DNA is packaged into capsids within the nucleus, which
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Navigating Novel Viral Challenges: Understanding, Tracking, and Mitigating Emerging Threats Microorganisms (IF 4.5) Pub Date : 2024-04-17 Francesco Branda, Massimo Ciccozzi
The emergence of new viral threats continues to pose significant challenges to global health security [...]
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Experimental Susceptibility of Nyssomyia antunesi and Lutzomyia longipalpis (Psychodidae: Phlebotominae) to Leishmania (Viannia) lainsoni and L. (V.) lindenbergi (Trypanosomatidae: Leishmaniinae) Microorganisms (IF 4.5) Pub Date : 2024-04-17 Yetsenia del Valle Sánchez Uzcátegui, Fernando Tobias Silveira, Thais Gouvea de Morais, Rodrigo Ribeiro Furtado, Thiago Vasconcelos dos Santos, Marinete Marins Póvoa
The present work assessed the experimental susceptibility of Nyssomyia antunesi and Lutzomyia longipalpis to Leishmania (Viannia) lainsoni and L. (V.) lindenbergi. A L. (Leishmania) chagasi–Lu. longipalpis combination was used as a susceptible control. Wild-caught Ny. antunesi and laboratory-bred Lu. longipalpis were membrane-fed on blood with a 5 × 106/mL log-phase promastigote culture suspension
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Long-Term Application of Biochar Mitigates Negative Plant–Soil Feedback by Shaping Arbuscular Mycorrhizal Fungi and Fungal Pathogens Microorganisms (IF 4.5) Pub Date : 2024-04-17 Mohamed Idbella, Silvia Baronti, Francesco Primo Vaccari, Ahmed M. Abd-ElGawad, Giuliano Bonanomi
Negative plant–soil feedback (PSF) arises when localized accumulations of pathogens reduce the growth of conspecifics, whereas positive PSF can occur due to the emergence of mutualists. Biochar, a carbon-rich material produced by the pyrolysis of organic matter, has been shown to modulate soil microbial communities by altering their abundance, diversity, and activity. For this reason, to assess the
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Enterocytozoon bieneusi in Wild Rats and Shrews from Zhejiang Province, China: Occurrence, Genetic Characterization, and Potential for Zoonotic Transmission Microorganisms (IF 4.5) Pub Date : 2024-04-17 Ting Zhang, Kuai Yu, Junchen Xu, Wenjie Cao, Yiqing Wang, Jiayan Wang, Liyuting Zhou, Jiani Chen, Huicong Huang, Wei Zhao
Globally, Enterocytozoon bieneusi has been detected in humans and various animal hosts. Wild rats and shrews have the potential to act as carriers of E. bieneusi, facilitating the parasite’s transmission to humans and domestic animals. We aimed to investigate the prevalence of E. bieneusi in 652 wild rats and shrews from Zhejiang Province, China, by amplifying the internal transcribed spacer (ITS)
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Associations of Fecal Microbiota with Ectopic Fat in African Caribbean Men Microorganisms (IF 4.5) Pub Date : 2024-04-17 Curtis Tilves, Noel T. Mueller, Joseph M. Zmuda, Allison L. Kuipers, Barbara Methé, Kelvin Li, John Jeffrey Carr, James G. Terry, Victor Wheeler, Sangeeta Nair, Iva Miljkovic
Objective: The gut microbiome has been associated with visceral fat (VAT) in European and Asian populations; however, associations with VAT and with ectopic fats among African-ancestry individuals are not known. Our objective was to investigate cross-sectional associations of fecal microbiota diversity and composition with VAT and ectopic fat, as well as body mass index (BMI), among middle-aged and
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Contribution of the Type III Secretion System (T3SS2) of Vibrio parahaemolyticus in Mitochondrial Stress in Human Intestinal Cells Microorganisms (IF 4.5) Pub Date : 2024-04-17 Nicolás Plaza, Diliana Pérez-Reytor, Gino Corsini, Katherine García, Ítalo M. Urrutia
Vibrio parahaemolyticus is an important human pathogen that is currently the leading cause of shellfish-borne gastroenteritis in the world. Particularly, the pandemic strain has the capacity to induce cytotoxicity and enterotoxicity through its Type 3 Secretion System (T3SS2) that leads to massive cell death. However, the specific mechanism by which the T3SS2 induces cell death remains unclear and
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Effects of Graphene-Based Nanomaterials on Microorganisms and Soil Microbial Communities Microorganisms (IF 4.5) Pub Date : 2024-04-17 Huifen Cao, Xiao Zhang, Haiyan Wang, Baopeng Ding, Sai Ge, Jianguo Zhao
The past decades have witnessed intensive research on the biological effects of graphene-based nanomaterials (GBNs) and the application of GBNs in different fields. The published literature shows that GBNs exhibit inhibitory effects on almost all microorganisms under pure culture conditions, and that this inhibitory effect is influenced by the microbial species, the GBN’s physicochemical properties
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Extended Reality Head-Mounted Displays Are Likely to Pose a Significant Risk in Medical Settings While Current Classification Remains as Non-Critical Microorganisms (IF 4.5) Pub Date : 2024-04-17 Adrian Goldsworthy, Matthew Olsen, Andy Koh, Thibaut Demaneuf, Gobinddeep Singh, Reem Almheiri, Brendan Chapman, Shaima Almazrouei, Rose Ghemrawi, Abiola Senok, Simon McKirdy, Rashed Alghafri, Lotti Tajouri
Extended reality (XR) devices, including virtual and augmented reality head-mounted displays (HMDs), are increasingly utilised within healthcare to provide clinical interventions and education. Currently, XR devices are utilised to assist in reducing pain and improving psychological outcomes for immunocompromised patients in intensive care units, palliative care environments and surgical theatres.
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Molecular and Serological Detection of Vector-Borne Pathogens Responsible for Equine Piroplasmosis in Europe between 2008 and 2021 Microorganisms (IF 4.5) Pub Date : 2024-04-17 Carla Wiebke Axt, Andrea Springer, Christina Strube, Clarissa Jung, Torsten J. Naucke, Elisabeth Müller, Ingo Schäfer
Equine piroplasmosis (EP) is caused by Theileria (T.) equi and/or Babesia (B.) caballi. The aim was to assess the percentage of positive test results for EP in horses in Europe and to identify risk factors for pathogen contact/infection. This study included results from PCR and competitive enzyme-linked immunosorbent assay testing requested by European veterinarians between 2008 and 2021. Binary bivariate
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Difference in the Effect of Applying Bacillus to Control Tomato Verticillium Wilt in Black and Red Soil Microorganisms (IF 4.5) Pub Date : 2024-04-15 Zhenhua Guo, Ziyu Lu, Zhongwang Liu, Wei Zhou, Shuangyu Yang, Jiayan Lv, Mi Wei
In practical applications, the effectiveness of biological control agents such as Bacillus is often unstable due to different soil environments. Herein, we aimed to explore the control effect and intrinsic mechanism of Bacillus in black soil and red soil in combination with tomato Verticillium wilt. Bacillus application effectively controlled the occurrence of Verticillium wilt in red soil, reducing
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Randomised, Placebo-Controlled Investigation of the Impact of Probiotic Consumption on Gut Microbiota Diversity and the Faecal Metabolome in Seniors Microorganisms (IF 4.5) Pub Date : 2024-04-15 Gabriella C. van Zanten, Anne Lundager Madsen, Christian C. Yde, Lukasz Krych, Nicolas Yeung, Markku T. Saarinen, Witold Kot, Henrik Max Jensen, Morten A. Rasmussen, Arthur C. Ouwehand, Dennis S. Nielsen
Aging has been associated with a changed composition and function of the gut microbiota (GM). Here, we investigate the effects of the multi-strain probiotic HOWARU® Restore on GM composition and function in seniors. Ninety-eight healthy adult volunteers aged ≥75 years were enrolled in a randomised, double-blinded intervention (NCT02207140), where they received HOWARU Restore (1010 CFU) or the placebo
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Variations and Interseasonal Changes in the Gut Microbial Communities of Seven Wild Fish Species in a Natural Lake with Limited Water Exchange during the Closed Fishing Season Microorganisms (IF 4.5) Pub Date : 2024-04-16 Yangyang Liang, Zijia Wang, Na Gao, Xiaoxue Qi, Juntao Zeng, Kai Cui, Wenxuan Lu, Shijie Bai
The gut microbiota of fish is crucial for their growth, development, nutrient uptake, physiological balance, and disease resistance. Yet our knowledge of these microbial communities in wild fish populations in their natural ecosystems is insufficient. This study systematically examined the gut microbial communities of seven wild fish species in Chaohu Lake, a fishing-restricted area with minimal water
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Insights on Pseudomonas aeruginosa Carbohydrate Binding from Profiles of Cystic Fibrosis Isolates Using Multivalent Fluorescent Glycopolymers Bearing Pendant Monosaccharides Microorganisms (IF 4.5) Pub Date : 2024-04-16 Deborah L. Chance, Wei Wang, James K. Waters, Thomas P. Mawhinney
Pseudomonas aeruginosa contributes to frequent, persistent, and, often, polymicrobial respiratory tract infections for individuals with cystic fibrosis (CF). Chronic CF infections lead to bronchiectasis and a shortened lifespan. P. aeruginosa expresses numerous adhesins, including lectins known to bind the epithelial cell and mucin glycoconjugates. Blocking carbohydrate-mediated host–pathogen and intra-biofilm