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Jellyfish protein hydrolysates: Multifunctional bioactivities unveiled in the battle against diabetes, inflammation, and bacterial pathogenesis Microb. Pathog. (IF 3.8) Pub Date : 2024-04-18 Noora Barzkar, Pinchuta Bunphueak, Phumin Chamsodsai, Pratchaya Muangrod, Benjawan Thumthanaruk, Vilai Rungsardthong, Lueacha Tabtimmai
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Comparative analysis of the intestinal flora of BmNPV-resistant and BmNPV-sensitive silkworm varieties Microb. Pathog. (IF 3.8) Pub Date : 2024-04-16 Xu Yang, Pai Liu, Haodong Yu, Min Ling, Mingzhen Ma, Qiang Wang, Xudong Tang, Zhongyuan Shen, Yiling Zhang
nucleopolyhedrovirus (BmNPV) is a very common and infectious virus that affects silkworms and hinders silk production. To investigate the intestinal flora of BmNPV-resistant and BmNPV-sensitive silkworm varieties, 16 S rDNA high-throughput sequencing was performed. The results of the cluster analysis showed that the intestinal flora of the resistant silkworm variety was more abundant than that of the
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Establishment and application of a quadruplex real-time RT-qPCR assay for differentiation of TGEV, PEDV, PDCoV, and PoRVA Microb. Pathog. (IF 3.8) Pub Date : 2024-04-16 Hechao Zhu, Geng Wang, Xiangzu Liu, Wenqing Wu, Teng Yu, Weichao Zhang, Xiangdong Liu, Guofu Cheng, Liuqing Wei, Lumei Ni, Zhong Peng, Xiangmin Li, Dequan Xu, Ping Qian, Pin Chen
Porcine viral diarrhea is a common ailment in clinical settings, causing significant economic losses to the swine industry. Notable culprits behind porcine viral diarrhea encompass transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and porcine rotavirus-A (PoRVA). Co-infections involving the viruses are a common occurrence in clinical
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Investigating the in vivo biocontrol and growth-promoting efficacy of Bacillus sp. and Pseudomonas fluorescens against olive knot disease Microb. Pathog. (IF 3.8) Pub Date : 2024-04-16 Avin Omer Ali, Hayman Kakakhan Awla, Tavga Sulaiman Rashid
Olive knot disease, caused by , poses a significant threat to olive cultivation, necessitating sustainable alternatives to conventional chemical control. This study investigates the biocontrol effectiveness of sp. (Og2) and (Oq5), alone and combined, against olive knot disease. Olive plants were sprayed with 5 ml of the bacteria until uniformly wet, with additional application to the soil surface.
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Oral bacterial insights from a comparative study between healthy and comorbid diseased human individuals Microb. Pathog. (IF 3.8) Pub Date : 2024-04-16 Anand Ravichandran, Jothilingam Sivapackiam, Saravanan Periasamy
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Exploring the antibiofilm and toxicity of tin oxide nanoparticles: Insights from in vitro and in vivo investigations Microb. Pathog. (IF 3.8) Pub Date : 2024-04-13 Soghra Nashath Omer, Venkatkumar Shanmugam
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Biology of tongue coating in different disease stages of RA and its value in disease progression Microb. Pathog. (IF 3.8) Pub Date : 2024-04-12 Qian Liu, Kangle Shi, Yunjing Bai, Fangyan Yang, Cong Lei, Xiaocong Wang, Ying Hu, Shiyao Wang, Ruikun Wang, Yuefan Yu, Xiaoyu Liu, Xinping Yu, Lingling Zhang, Ling Tang, Suqian Li, Qinggang Meng
To assess and compare the composition of tongue coating microbiota among patients at different stages of rheumatoid arthritis (RA). A total of 47 patients diagnosed with RA, as per the American College of Rheumatology criteria, and 10 healthy individuals were enrolled in this study. The RA patients were stratified considering their Disease Activity Score 28 (DAS28), a composite measure based on the
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Adhesion of Klebsiella oxytoca to bladder or lung epithelial cells is promoted by the presence of other opportunistic pathogens Microb. Pathog. (IF 3.8) Pub Date : 2024-04-09 Adeliia Giliazeva, Yaw Akosah, Jonas Noack, Ayslu Mardanova
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Probiotics from kefir: Evaluating their immunostimulant and antioxidant potential in the carpet shell clam (Ruditapesdecussatus) Microb. Pathog. (IF 3.8) Pub Date : 2024-04-06 Chalbia Mansour, Fadia Ben Taheur, Sihem Safta Skhiri, Mourad Jridi, Dalila Saidane Mosbahi, Nacim Zouari
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Identification and characterization of silver nanoparticles from Erythrina indica and its antioxidant and Uropathogenic antimicrobial properties Microb. Pathog. (IF 3.8) Pub Date : 2024-04-03 Azhagu Madhavan Sivalingam, Arjun Pandian
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Infectious bronchitis virus (IBV) triggers autophagy to enhance viral replication by activating the VPS34 complex Microb. Pathog. (IF 3.8) Pub Date : 2024-04-02 Gaojie Song, Cuiling Zhang, Xiaoyang Yu, Jiaqi Li, Qinlei Fan, Fei Liu, Qinghao He, Chao Shang, Xiao Li
Autophagy plays an important role in the lifecycle of viruses. However, there is currently a lack of systematic research on the relationship between Infectious Bronchitis Virus (IBV) and autophagy. This study aims to investigate the impact of IBV on autophagy and the role of autophagy in viral replication. We observed that IBV infection increased the expression of microtubule-associated protein 1 light
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Analysis of factors influencing the risk of secondary infection in patients colonized or infected with multidrug-resistant Gram-negative bacteria following hospitalization Microb. Pathog. (IF 3.8) Pub Date : 2024-04-01 Min Xu, Jing Zeng
We seek to investigate the multifaceted factors influencing secondary infections in patients with multidrug-resistant Gram-negative bacteria (MDR-GNB) colonization or infection post-hospitalization. A total of 100 patients with MDR-GNB colonization or infection were retrospectively reviewed, encompassing those admitted to both the general ward and intensive care unit of our hospital from August 2021
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Distribution and virulence of Escherichia coli harboring cyclomodulins and supplementary virulence genes isolates from clinical and environmental samples Microb. Pathog. (IF 3.8) Pub Date : 2024-03-30 Nora DeLira-Bustillos, Uriel A. Angulo-Zamudio, Nidia Leon-Sicairos, Hector Flores-Villaseñor, Jorge Velazquez-Roman, Gabriela Tapia-Pastrana, Francisco A. Martínez-Villa, Rafael Velázquez-Cruz, Jorge Salmerón, Samuel Canizales-Quinteros, Adrian Canizalez-Roman
This study aimed to determine the prevalence of cyclomodulins (, , and ) in () isolated from clinical and environmental samples, the presence of supplementary virulence genes (SVG), antibiotic resistance, and cytotoxicity. 413 were isolated from clinical (stool from obese subjects, normal weight subjects, children with diarrhea, and children without diarrhea; and urine from pregnant and non-pregnant
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The colonization factor CS6 of enterotoxigenic Escherichia coli contributes to host cell invasion Microb. Pathog. (IF 3.8) Pub Date : 2024-03-29 Alafate Ayibieke, Takeaki Wajima, Shigeyuki Kano, Nabendu Sekhar Chatterjee, Takashi Hamabata
Enterotoxigenic (ETEC) is one of the main causes of diarrhea in children and travelers in low-income regions. The virulence of ETEC is attributed to its heat-labile and heat-stable enterotoxins, as well as its colonization factors (CFs). CFs are essential for ETEC adherence to the intestinal epithelium. However, its invasive capability remains unelucidated. In this study, we demonstrated that the CS6-positive
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Porcine HERC6 acts as major E3 ligase for ISGylation and is auto-ISGylated for effective ISGylation in porcine Microb. Pathog. (IF 3.8) Pub Date : 2024-03-29 Cuiyu Ba, Chaonan Tian, Xueying Bo, Chang Xu, Yi Zhang, Xiangyu Sun, Yuchen Nan, Chunyan Wu
Interferon-stimulated gene product 15 (ISG15) can be conjugated to substrates through ISGylation. Currently, the E3 ligase for porcine ISGylation remains unclear. Here, we identified porcine HERC5 and HERC6 (pHERC5/6) as ISGylation E3 ligases with pHERC6 acting as a major one by reconstitution of porcine ISGylation system in HEK-293 T cell via co-transfecting E1, E2 and porcine ISG15(pISG15) genes
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Optimized production of full-length PCV2d virus-like particles in Escherichia coli: A cost-effective and high-yield approach for potential vaccine antigen development Microb. Pathog. (IF 3.8) Pub Date : 2024-03-29 Chengxin Zhang, Fang He, Nianfeng Li, Wei Du, Jianxin Wen, Xiaoyan Wu, Jianli Shi, Chen Li, Chang Liu, Shaojian Xu, Hong Han, Nataliia Hrabchenko, Xianjie Han, Jun Li
Porcine circovirus type 2 (PCV2) is a globally prevalent infectious pathogen affecting swine, with its capsid protein (Cap) being the sole structural protein critical for vaccine development. Prior research has demonstrated that PCV2 Cap proteins produced in () can form virus-like particles (VLPs) in vitro, and nuclear localization signal peptides (NLS) play a pivotal role in stabilizing PCV2 VLPs
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Molecular characteristics and biofilm formation capacity of nontypeable Haemophilus influenza strains isolated from lower respiratory tract in children Microb. Pathog. (IF 3.8) Pub Date : 2024-03-26 Jiying Xiao, Lin Su, Xiya Chen, Shumin Huang, Mingming Zhou, Zhimin Chen
With the widespread introduction of the Hib conjugate vaccine, Nontypeable Haemophilus influenzae (NTHi) has emerged as the predominant strain globally. NTHi presents a significant challenge as a causative agent of chronic clinical infections due to its high rates of drug resistance and biofilm formation. While current research on NTHi biofilms in children has primarily focused on upper respiratory
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Rapamycin improves the long-term T-cell memory and protective efficacy of tuberculosis subunit vaccine Microb. Pathog. (IF 3.8) Pub Date : 2024-03-26 Hongxia Niu, Chunxiang Bai, Bingdong Zhu, Ying Zhang
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Genetic characteristics of Blastocystis sp. in cattle from Hebei Province, China Microb. Pathog. (IF 3.8) Pub Date : 2024-03-24 Yuxin Sun, Huizhu Nan, Chao Zhang, Xin Yang, Yan Zhao, Gang Feng, Lei Ma
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Discovery of novel dihydronaphthalene—imidazole ligands as potential inhibitors of Staphylococcus aureus multidrug resistant NorA efflux pump: A combination of experimental and in silico molecular docking studies Microb. Pathog. (IF 3.8) Pub Date : 2024-03-21 Asif A Malik, Nisar A. Dangroo, Parminder Kaur, Shobit Attery, Manzoor A Rather, Abrar Khan, Tabassum Ara, Hemraj Nandanwar
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Development of a double antibody sandwich ELISA method for the quantitative detection of serum C-reactive protein based on nanobody Microb. Pathog. (IF 3.8) Pub Date : 2024-03-21 Xin Liu, Changjiang Wang, Yu Bai, Weichen Wang, Yuchen Han, Shu Cai, Jiajia An, Guanggang Qu
In this study, we successfully developed a nanobody-based double antibody sandwich ELISA kit for the detection of clinical serum -reactive protein (CRP) by using two novel CRP specific nanobodies.
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Bacterial-derived sialidases inhibit porcine rotavirus OSU replication by interfering with the early steps of infection Microb. Pathog. (IF 3.8) Pub Date : 2024-03-19 Yucheng Huang, Qian Zhu, Yongqiang Wang, Kui Zhu
Rotavirus infections in suckling and weaning piglets cause severe dehydration and death, resulting in significant economic losses in the pig breeding industry. With the continuous emergence of porcine rotavirus (PoRV) variants and poor vaccine cross-protection among various genotypes, there is an urgent need to develop alternative strategies such as seeking effective antiviral products from nature
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Unlocking bacterial defense: Exploring the potent inhibition of NorA efflux pump by coumarin derivatives in Staphylococcus aureus Microb. Pathog. (IF 3.8) Pub Date : 2024-03-17 Ana Luíza A.R. Martin, Raimundo Luiz Silva Pereira, Janaína Esmeraldo Rocha, Pablo A.M. Farias, Thiago S. Freitas, Francisco Rodrigo de Lemos Caldas, Fernando G. Figueredo, Nadghia Figueiredo Leite Sampaio, Cícera Datiane de Morais Oliveira-Tintino, Saulo Relison Tintino, Gabriel Costa A. da Hora, Maria Carolina Pacheco Lima, Irwin Rose A. de Menezes, Diogo T. Carvalho, Henrique D.M. Coutinho, Marta
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Application of cuminaldehyde and ciprofloxacin for the effective control of biofilm assembly of Pseudomonas aeruginosa: A combinatorial study Microb. Pathog. (IF 3.8) Pub Date : 2024-03-16 Moumita Malik, Sharmistha Das, Poulomi Chakraborty, Payel Paul, Ritwik Roy, Anirban Das Gupta, Sarita Sarkar, Sudipta Chatterjee, Alakesh Maity, Monikankana Dasgupta, Ranojit Kumar Sarker, Prosun Tribedi
is widely associated with biofilm-mediated antibiotic resistant chronic and acute infections which constitute a persistent healthcare challenges. Addressing this threat requires exploration of novel therapeutic strategies involving the combination of natural compounds and conventional antibiotics. Hence, our study has focused on two compounds; cuminaldehyde and ciprofloxacin, which were strategically
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Good girl goes bad: Understanding how gut commensals cause disease Microb. Pathog. (IF 3.8) Pub Date : 2024-03-16 Priyankar Dey
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Antagonistic activity and mechanism of Bacillus subtilis CG-6 suppression of root rot and growth promotion in Alfalfa Microb. Pathog. (IF 3.8) Pub Date : 2024-03-15 Jiaxin Chen, Run Cai, Lu Tang, Dan Wang, Ruiwei Lv, Changhong Guo
Root rot is a common disease, that severely affects the yield and quality of alfalfa. Biocontrol is widely used to control plant diseases caused by pathogenic fungi, however, biocontrol strains for alfalfa root rot are very limited. In this study, a CG-6 strain with a significant biocontrol effect on alfalfa root rot was isolated. CG-6 secretes antibacterial enzymes and siderophore, phosphate solubilization
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Acanthopanax senticosus cultures fermented by Lactobacillus rhamnosus enhanced immune response through improvement of antioxidant activity and inflammation in crucian carp (Carassius auratus) Microb. Pathog. (IF 3.8) Pub Date : 2024-03-15 Yi-Han Ma, Yu-Di Sheng, Di Zhang, Jun-Tong Liu, Ye Tian, Hui Li, Xiao-Fei Li, Na Li, Peng Sun, Shahrood Ahmed Siddiqui, Wu-wen Sun, Lei Zhang, Xiao-Feng Shan, Chun-Feng Wang, Ai-Dong Qian, Dong-Xing Zhang
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Enhanced control efficacy of Bacillus subtilis NM4 via integration of chlorothalonil on potato early blight caused by Alternaria solani Microb. Pathog. (IF 3.8) Pub Date : 2024-03-14 Jun Su Noh, Seo Hyun Hwang, Chaw Ei Htwe Maung, Jeong-Yong Cho, Kil Yong Kim
Early blight caused by is a common foliar disease of potato around the world, and serious infections result in reduced yields and marketability due to infected tubers. The major aim of this study is to figure out the synergistic effect between microorganism and fungicides and to evaluate the effectiveness of NM4 in the control of early blight in potato. Based on its colonial morphology and a 16S rRNA
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Macrophage immunophenotypes in Jorge Lobo’s disease and lepromatous leprosy- A comparative study Microb. Pathog. (IF 3.8) Pub Date : 2024-03-13 BM Sasso, AFA Vallarelli, PS Rosa, AFF Belone, PENF Velho, Maria Leticia Cintra
Jorge Lobo's disease (JLD) and lepromatous leprosy (LL) share several clinical, histological and immunological features, especially a deficiency in the cellular immune response. Macrophages participate in innate and adaptive inflammatory immune responses, as well as in tissue regeneration and repair. Macrophage function deficiency results in maintenance of diseases. M1 macrophages produce pro-inflammatory
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Biological activity of silver nanoparticles synthesized using viticultural waste Microb. Pathog. (IF 3.8) Pub Date : 2024-03-12 Anna Miškovská, Jana Michailidu, Irena Jarošová Kolouchová, Ludovica Barone, Rosalba Gornati, Aurora Montali, Gianluca Tettamanti, Francesca Berini, Flavia Marinelli, Jan Masák, Alena Čejková, Olga Maťátková
This research paper presents a novel approach to the green synthesis of silver nanoparticles (AgNPs) using viticultural waste, allowing to obtain NP dispersions with distinct properties and morphologies (monodisperse and polydisperse AgNPs, referred to as mAgNPs and pAgNPs) and to compare their biological activities. Our synthesis method utilized the ethanolic extract of pruning residues, resulting
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Isolation and characterization of a G9P[23] porcine rotavirus strain AHFY2022 in China Microb. Pathog. (IF 3.8) Pub Date : 2024-03-11 Jianxin Wang, Jinzhu Zhou, Xuejiao Zhu, Xianyu Bian, Nan Han, Baochao Fan, Laqiang Gu, Xi Cheng, Sufen Li, Ran Tao, Jizong Li, Xuehan Zhang, Bin Li
Rotavirus group A (RVA) is a main pathogen causing diarrheal diseases in humans and animals. Various genotypes are prevalent in the Chinese pig herd. The genetic diversity of RVA lead to distinctly characteristics. In the present study, a porcine RVA strain, named AHFY2022, was successfully isolated from the small intestine tissue of piglets with severe diarrhea. The AHFY2022 strain was identified
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Identification, characterization and complete genome analysis of a Vibrio anguillarum isolated from Sebastes schlegelii Microb. Pathog. (IF 3.8) Pub Date : 2024-03-11 Zeyu Li, Yungui Sun, Ruiming Tan, Yingli Gao
is an important fish pathogen in mariculture, which can infect fish with great economic losses. In this study, a isolated from was named VA1 and was identified and characterized from aspects of morphology, physiological and biochemical characteristics, 16SRNA, virulence genes, drug sensitivity, and extracellular enzyme activity. At the same time, The VA1 was investigated at the genomic level. The results
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Molecular insights into the variability and pathogenicity of Fusarium odoratissimum, the causal agent of Panama wilt disease in banana Microb. Pathog. (IF 3.8) Pub Date : 2024-03-06 Padma Tamang, Pradeep Kumar, Arpit Chauhan, Satyam Rastogi, Suchi Srivastava, Satya Narayan Jena
Fusarium wilt or Panama disease of banana caused by the hemibiotroph fungus, , also known as f.sp. Tropical Race 4 is a serious threat to banana production worldwide. Being the world's largest grower and the origins of bananas in its northeast region, India is particularly vulnerable to this deadly fungus. In the present study, a total of 163 isolates from infected banana were characterized for their
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Attenuation of Las/Rhl quorum sensing regulated virulence and biofilm formation in Pseudomonas aeruginosa PAO1 by Artocarpesin Microb. Pathog. (IF 3.8) Pub Date : 2024-03-05 Mahima S. Mohan, Simi Asma Salim, Sampathkumar Ranganathan, Paramanantham Parasuraman, V.T. Anju, Dinakara Rao Ampasala, Madhu Dyavaiah, Jung-Kul Lee, Siddhardha Busi
The emergence of multidrug resistance and increased pathogenicity in microorganisms is conferred by the presence of highly synchronized cell density dependent signalling pathway known as quorum sensing (QS). The QS hierarchy is accountable for the secretion of virulence phenotypes, biofilm formation and drug resistance. Hence, targeting the QS phenomenon could be a promising strategy to counteract
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Extracellular vesicles from Candida albicans modulate immune cells function and play a protective role in fungal keratitis Microb. Pathog. (IF 3.8) Pub Date : 2024-03-02 Huijin Duan, Fanyue Meng, Xing Liu, Pingli Qi, Xudong Peng, Cui Li, Qian Wang, Guiqiu Zhao, Jing Lin
Fungal keratitis (FK) is a highly blinding infectious corneal disease caused by pathogenic fungi. () is one of the main pathogens of fungal keratitis. Extracellular vesicles (EVs), lipid bilayer compartments released by almost all living cells, including fungi, have garnered attention for their role in pathogenic microbial infection and host immune responses in recent years. Studies have reported that
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Mechanistic insights on the antibacterial action of the kyotorphin peptide derivatives revealed by in vitro studies and Galleria mellonella proteomic analysis Microb. Pathog. (IF 3.8) Pub Date : 2024-03-02 Vitor M. de Andrade, Vitor D.M. de Oliveira, Uilla Barcick, Vasanthakumar G. Ramu, Montserrat Heras, Eduard R. Bardají, Miguel A.R.B. Castanho, André Zelanis, Aline Capella, Juliana C. Junqueira, Katia Conceição
The selected kyotorphin derivatives were tested to improve their antimicrobial and antibiofilm activity. The antimicrobial screening of the KTP derivatives were ascertained in the representative strains of bacteria, including , , and . Kyotorphin derivatives, KTP-NH, KTP–NH–DL, IbKTP, IbKTP-NH, MetKTP-DL, MetKTP-LD, were designed and synthesized to improve lipophilicity and resistance to enzymatic
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Dual-RPA assay for rapid detection and differentiation of E.granulosus and E.multilocularis Microb. Pathog. (IF 3.8) Pub Date : 2024-02-29 Shu Cai, Changjiang Wang, Fengrong Tian, Mengxiao Liu, Hongbin Yan, Zaiyan Xu, Guanggang Qu
(Eg) and (Em) are the two most widely prevalent types of echinococcosis. Several diagnostic methods have been developed for detecting Eg and Em. However, some limitations, such as being time-consuming, needing expensive instruments, or exhibiting low sensitivity, make these methods unsuitable for on-site detection. In this study, a dual-RPA assay was established to detect and differentiate Eg and Em
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Platycodon grandiflorus polysaccharide inhibits the inflammatory response of 3D4/21 cells infected with PCV2 Microb. Pathog. (IF 3.8) Pub Date : 2024-02-28 Xiaocheng Guo, Ximan Zhao, Linjue Li, Menglin Jiang, Aiqin Zhou, Yifan Gao, Pimiao Zheng, Jianzhu Liu, Xiaona Zhao
Porcine circovirus type 2 (PCV2) infection cause multi-systemic inflammation in pigs. polysaccharide (PGPS) has been reported to have the effects of immune regulation and disease resistance. Nevertheless, the role and mechanism of PGPS in the inflammatory response of 3D4/21 cells induced by PCV2 infection remain unclear. The present study aims to investigate effects of PGPS on inflammatory response
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Antibacterial activity and mechanism of cell-free supernatants of Lacticaseibacillus paracasei against Propionibacterium acnes Microb. Pathog. (IF 3.8) Pub Date : 2024-02-28 Jiahui Xu, Xiaoyong Chen, Jiajia Song, Chen Wang, Weiping Xu, Han Tan, Huayi Suo
() is an anaerobic and gram-positive bacterium involved in the pathogenesis and inflammation of acne vulgaris. This study particularly focuses on the antimicrobial effect of LPH01 against . , a bacterium that causes acne vulgaris. Fifty-seven strains were tested for their ability to inhibit growth employing the Oxford Cup and double dilution methods. The cell-free supernatant (CFS) of LPH01 demonstrated
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Impacts of intake of trichothecenes (Fusarium sporotrichioides) for dairy calves: Effects on animal growth, oxidative and inflammatory response Microb. Pathog. (IF 3.8) Pub Date : 2024-02-28 Marcela Machado, Luisa Nora, Tayse Burger Neto Zanin, Bianca F. Bissacotti, Vera M. Morsch, Marcelo Vedovatto, Giovana Pelisser, Ricardo E. Mendes, Alessandro C. Galvão, Caroline Sefrin Speroni, Eduardo M. Gloria, Murilo F. Rodrigues, Roger Wagner, Lenita M. Stefani, Aleksandro S. da Silva
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Pepper defense against Ralstonia solanacearum and High-temperature stress is positively regulated by CaMYB59 Microb. Pathog. (IF 3.8) Pub Date : 2024-02-28 Ali Noman, Khairiah Mubarak Alwutayd, Muhammad Aqeel, Ansar Hussain, Muhammad Qasim, Rahmah N. Al-Qthanin, Mohammed O. Alshaharni, Fahad Mohammed Alzuaibr, Maryam M. Alomran
We have functionally evaluated a transcription factor for its role in pepper immune responses to attack and high temperature-high humidity (HTHH). Exposure to . inoculation (RSI) and HTHH resulted in up-regulation of this nucleus-localized TF. Function of this TF was confirmed by performing loss of function assay of CaMYB59 by VIGS (virus-induced gene silencing). Plants with silenced CaMYB59 displayed
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Enhancement of Streptomyces thinghirensis WAE1 for production of bioactive metabolites under different optimization strategies Microb. Pathog. (IF 3.8) Pub Date : 2024-02-28 Mohamed E. Osman, Amany A. Abo Elnasr, Eslam T. Mohamed, Ahmed H.I. Faraag
Isolation of novel bioactive metabolites from strains is a promising source for drug discovery. However, conventional screening approaches have limitations in identifying new leads due to redundant discoveries. Optimization of culture conditions is important but traditionally optimized one factor at a time, failing to consider interactions. This study addressed these gaps by enhancing metabolite production
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Piperine, a phytochemical prevents the biofilm city of methicillin-resistant Staphylococcus aureus: A biochemical approach to understand the underlying mechanism Microb. Pathog. (IF 3.8) Pub Date : 2024-02-27 Sharmistha Das, Moumita Malik, Debabrata Ghosh Dastidar, Ritwik Roy, Payel Paul, Sarita Sarkar, Poulomi Chakraborty, Alakesh Maity, Monikankana Dasgupta, Anirban Das Gupta, Sudipta Chatterjee, Ranojit Kumar Sarker, Debasish Maiti, Prosun Tribedi
Methicillin-resistant (MRSA), a drug-resistant human pathogen causes several nosocomial as well as community-acquired infections involving biofilm machinery. Hence, it has gained a wide interest within the scientific community to impede biofilm-induced MRSA-associated health complications. The current study focuses on the utilization of a natural bioactive compound called piperine to control the biofilm
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Br - nanoconjugate enhances the antibacterial efficacy of nimboloide against Flavobacterium columnare infection in Labeo rohita: A nanoinformatics approach Microb. Pathog. (IF 3.8) Pub Date : 2024-02-27 Sanjana Mishra, Prekshi Garg, Shilpi Srivastava, Prachi Srivastava
The bacterial pathogen, causes columnaris disease in globally. Major effects of this bacterial infection include skin rashes and gill necrosis. Nimbolide, the key ingredient of the leaf extract of possesses anti-bacterial properties effective against many microorganisms. Nano-informatics plays a promising role in drug development and its delivery against infections caused by multi-drug-resistant bacteria
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Antimicrobial activity of probiotic bacteria-mediated cadmium oxide nanoparticles against fish pathogens Microb. Pathog. (IF 3.8) Pub Date : 2024-02-24 Shanmugam Rajeshkumar, Santhoshkumar Jayakodi, M. Tharani, Naiyf S. Alharbi, Muthu Thiruvengadam
The current research was designed to investigate the antibacterial activity of probiotic bacteria mediated cadmium oxide nanoparticles (CdO NPs) on common fish pathogenic bacteria like , , , and . . CdO NPs were synthesized using probiotic bacteria as follows: species with different precursor of cadmium sulfate concentrations (5, 10, and 20 mM). The average crystalline sizes of the CdO NPs were determined
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Δfur mutant as a potential live attenuated vaccine (LAV) candidate protects American eels (Anguilla rostrata) from Vibrio harveyi infection Microb. Pathog. (IF 3.8) Pub Date : 2024-02-23 Qijuan Wan, Shaowei Zhai, Minxia Chen, Ming Xu, Songlin Guo
The eel farming industry is highly susceptible to . Although various types of vaccines against have been investigated, there is limited research on decreasing the virulence of s through gene knockout and utilizing it as live attenuated vaccines (LAV). In this study, we aim to develop a LAV candidate against infection in American eels () using a ferric uptake regulator () gene mutant strain of (Δ mutant)
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Gut microbiome in diabetic retinopathy: A systematic review and meta-analysis Microb. Pathog. (IF 3.8) Pub Date : 2024-02-23 Shuang Zhao, Qi Yan, Wanjing Xu, Juanmei Zhang
Changes in the gut microbiome are linked with Type 2diabetes mellitus (T2DM) development, but alterations in patients with diabetic retinopathy (DR) are still being debated. To investigate the differences in biodiversity and relative abundance of gut microbiome between patients with DR and T2DM. A comprehensive search was performed in five electronic databases (PubMed, EMBASE, Cochrane Central Register
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Evaluation of long-term immune response in cattle to botulism using a recombinant E. coli bacterin formulated with Montanide™ ISA 50 and aluminum hydroxide adjuvants Microb. Pathog. (IF 3.8) Pub Date : 2024-02-21 Clovis Moreira Jr., Rafael R. Rodrigues, Carlos E.P. da Cunha, Rafael A. Donassolo, Marcos R.A. Ferreira, Paula F. Finger, Hanna G.S. Oliveira, Karoline P. da Cruz, Ângela N. Moreira, Felipe M. Salvarani, Fabricio R. Conceição
Botulism is a severe disease caused by potent botulinum neurotoxins (BoNTs) produced by . This disease is associated with high-lethality outbreaks in cattle, which have been linked to the ingestion of preformed BoNT serotypes C and D, emphasizing the need for effective vaccines. The potency of current commercial toxoids (formaldehyde-inactivated BoNTs) is assured through tests in guinea pigs according
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Deletion of speA and aroC genes impacts the pathogenicity of Vibrio anguillarum in spotted sea bass Microb. Pathog. (IF 3.8) Pub Date : 2024-02-21 Haisheng Xie, Yajuan Huang, Shengming Wang, Jingyuan Che, Tuyan Luo, Lekang Li, Baolong Bao
is one of the major pathogens responsible for bacterial infections in marine environments, causing significant impacts on the aquaculture industry. The misuse of antibiotics leads to bacteria developing multiple drug resistances, which is detrimental to the development of the fisheries industry. In contrast, live attenuated vaccines are gradually gaining acceptance and widespread recognition. In this
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Combined analysis of the microbiome, metabolome and transcriptome of silkie chickens in response to avian pathogenic E. coli (APEC) Microb. Pathog. (IF 3.8) Pub Date : 2024-02-20 Wenqing Li, Wanli Li, Qinghan Zhao, Pinhui Wu, Xinmeng Huang, Wei Jin, Bingxun Wang, Shengli Li, Wei Liu, Guozhi Zhang, Xiangtao Kang
Avian colibacillosis is a bacterial disease caused by avian pathogenic (APEC) that results in great losses in the poultry industry every year. Individual Silkie chickens of the same breed that are given the same feed in the same feeding conditions have different levels of resistance or susceptibility to APEC. Differences in gut microbes, gut metabolites, and gene expression in the spleen of APEC-resistant
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Comparative transcriptomics reveals common and strain-specific responses of human macrophages to infection with Mycobacterium tuberculosis and Mycobacterium bovis BCG Microb. Pathog. (IF 3.8) Pub Date : 2024-02-20 Pei Li, Yang Li, Cun Chuan Wang, Li Gang Xia
(MTB) and () are closely related pathogenic mycobacteria known to cause chronic pulmonary infections in both humans and animals. Despite sharing nearly identical genomes and virulence factors, these two bacteria display variations in host tropism, epidemiology, and clinical presentations. Bacillus Calmette–Guérin (BCG) is an attenuated strain of commonly utilized as a vaccine for tuberculosis (TB)
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Synthesis and characterization of marine seagrass (Cymodocea serrulata) mediated titanium dioxide nanoparticles for antibacterial, antibiofilm and antioxidant properties Microb. Pathog. (IF 3.8) Pub Date : 2024-02-20 Mohankumar Narayanan, Suganthi Srinivasan, Chackaravarthi Gnanasekaran, Govindan Ramachandran, Chenthis Kanisha Chelliah, Govindan Rajivgandhi, Muthuchamy Maruthupandy, Franck Quero, Wen-Jun Li, Gasim Hayder, Jamal M. Khaled, Arulraj Arunachalam, Natesan Manoharan
mediated titanium dioxide nanoparticles (TiO NPs) were successfully synthesized. The XRD pattern and FTIR spectra demonstrated the crystalline structure of TiO NPs and the presence of phenols, flavonoids and alkaloids in the extract. Further SEM revealed that TiO NPs has uniform structure and spherical in shape with their size ranged from 58 to 117 nm. Antibacterial activity of TiO NPs against methicillin-resistant
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Corrigendum to “Kosakonia cowanii, a new bacterial pathogen affecting foxtail millet (Setaria italica[L.]P. Beauv.) in China” [Microb Pathog 181 (2023) 106201] Microb. Pathog. (IF 3.8) Pub Date : 2024-02-20 Yanqing Han, Xi Gao, Guoli Huang, Yindong Chang, Haili Han, Jiao Zhu, Baojun Zhang
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A novel perspective on eugenol as a natural anti-quorum sensing molecule against Serratia sp. Microb. Pathog. (IF 3.8) Pub Date : 2024-02-20 Fakher Frikha, Marwa Jardak, Sami Aifa, Sami Mnif
is commonly noted to be an opportunistic pathogen and is often associated with nosocomial infections. In addition to its high antibiotic resistance, it exhibits a wide range of virulence factors that confer pathogenicity. Targeting quorum sensing (QS) presents a potential therapeutic strategy for treating bacterial infections caused by , as it regulates the expression of various virulence factors.
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Pre-clinical safety and toxicity assessment of Limosilactobacillus fermentum NCDC 400 in murine model Microb. Pathog. (IF 3.8) Pub Date : 2024-02-19 Basavaprabhu Haranahalli Nataraj, K. Jeevan, Ajay Kumar Dang, Ravinder Nagpal, Syed Azmal Ali, Pradip V. Behare
Comprehensive safety assessment of potential probiotic strains is crucial in the selection of risk-free strains for clinical translation. This study aimed to evaluate the biosafety of NCDC 400, a potential probiotic strain, using oral toxicity tests in a Swiss albino mouse model. Mice were orally gavaged with low (10 CFU/mouse/day) and high (10 CFU/mouse/day) doses of NCDC 400 for 14 (acute), 28 (subacute)
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Blood transcriptome analysis uncovered COVID-19–myocarditis crosstalk Microb. Pathog. (IF 3.8) Pub Date : 2024-02-17 Shuang Liang, Ying-Ying Zheng, Ying Pan
The condition of COVID-19-related myocarditis has emerged as a prominent contributor to COVID-19 mortality. As the epidemic persists, its incidence continues to rise. Despite ongoing efforts, the elucidation of COVID-19-related myocarditis underlying molecular mechanisms still requires further investigation. Hub genes for COVID-19-related myocarditis were screened by integrating gene expression profile
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Screening for immune biomarkers associated with infection or protection against Ehrlichia ruminantium by RNA-sequencing analysis Microb. Pathog. (IF 3.8) Pub Date : 2024-02-16 A. Pretorius, T. Nefefe, N. Thema, J. Liebenberg, H. Steyn, M. van Kleef
Heartwater is one of the most economically important tick-borne fatal diseases of livestock. The disease is caused by the bacteria transmitted by ticks. Although there is evidence that interferon-gamma controls growth and that cellular immune responses are protective, an effective recombinant vaccine for this disease is lacking. Analyses of markers associated with infection as well as protection will
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Macrophage migration inhibitory factor favors Neospora caninum infection in mice Microb. Pathog. (IF 3.8) Pub Date : 2024-02-16 Vanessa Resende Souza Silva, Caroline Martins Mota, Lydiane Parreira Maia, Flávia Batista Ferreira, Vanessa dos Santos Miranda, Neide Maria Silva, Eloísa A. Vieira Ferro, José Roberto Mineo, Tiago Wilson Patriarca Mineo
is a protozoan parasite with worldwide incidence, acting as a major cause of reproductive failures in ruminants and neuromuscular symptoms in dogs. Macrophage Migration Inhibitory Factor (MIF) is produced by several cell types and exhibits a central role in immune responses against intracellular pathogens. The present study aimed to comprehend the role of MIF in the relationship between and its host
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Evaluation of reference genes for gene expression analysis by real-time quantitative PCR (qPCR) in different tissues from mice infected by Ascaris suum Microb. Pathog. (IF 3.8) Pub Date : 2024-02-14 Jorge Lucas Nascimento Souza, Camila de Almeida Lopes, Thais Leal-Silva, Flaviane Vieira-Santos, Chiara Cássia Oliveira Amorim, Luiza de Lima Silva Padrão, Ana Rafaela Antunes Porto, Ricardo Toshio Fujiwara, Remo Castro Russo, Lilian Lacerda Bueno
Human ascariasis is the most prevalent helminth infection, affecting 445 million people worldwide. To better understand the impact of the immune system on the pathophysiology of individuals infected with , mice have been used as experimental models. The RT-qPCR technique is a critical auxiliary tool of investigation used to quantify mRNA levels. However, proper normalization using reference genes is