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Periodontitis, Dental Procedures, and Young-Onset Cryptogenic Stroke J. Dent. Res. (IF 7.6) Pub Date : 2024-04-16 J. Leskelä, J. Putaala, N. Martinez-Majander, L. Tulkki, M. Manzoor, S. Zaric, P. Ylikotila, R. Lautamäki, A. Saraste, S. Suihko, E. Könönen, J. Sinisalo, P.J. Pussinen, S. Paju
Periodontitis is associated with an increased risk of ischemic stroke, and the risk may be particularly high among young people with unexplained stroke etiology. Thus, we investigated in a case-control study whether periodontitis or recent invasive dental treatments are associated with young-onset cryptogenic ischemic stroke (CIS). We enrolled participants from a multicenter case-control SECRETO study
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Tongue-Coating Microbial and Metabolic Characteristics in Halitosis J. Dent. Res. (IF 7.6) Pub Date : 2024-04-16 Y. Zhang, K.L. Lo, A.N. Liman, X.P. Feng, W. Ye
Halitosis is a common oral condition, which leads to social embarrassment and affects quality of life. Cumulative evidence has suggested the association of tongue-coating microbiome with the development of intraoral halitosis. The dynamic variations of tongue-coating microbiota and metabolites in halitosis have not been fully elucidated. Therefore, the present study aimed to determine the tongue-coating
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Spatiotemporal Evolution of Developing Palate in Mice J. Dent. Res. (IF 7.6) Pub Date : 2024-04-15 B. Wang, Z. Zhang, J. Zhao, Y. Ma, Y. Wang, N. Yin, T. Song
The intricate formation of the palate involves a series of complex events, yet its mechanistic basis remains uncertain. To explore major cell populations in the palate and their roles during development, we constructed a spatiotemporal transcription landscape of palatal cells. Palate samples from C57BL/6 J mice at embryonic days 12.5 (E12.5), 14.5 (E14.5), and 16.5 (E16.5) underwent single-cell RNA
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Strategies for Improving Impaired Osseointegration in Compromised Animal Models J. Dent. Res. (IF 7.6) Pub Date : 2024-04-15 J. Deng, C. Van Duyn, D. J. Cohen, Z. Schwartz, B. D. Boyan
Implant osseointegration is reduced in patients with systemic conditions that compromise bone quality, such as osteoporosis, disuse syndrome, and type 2 diabetes. Studies using rodent models designed to mimic these compromised conditions demonstrated reduced bone-to-implant contact (BIC) or a decline in bone mineral density. These adverse effects are a consequence of disrupted intercellular communication
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The Alcohol Harm Paradox in Periodontitis J. Dent. Res. (IF 7.6) Pub Date : 2024-04-12 L.M. Oliveira, F.B. Zanatta, S.A. Costa, T.R. Pelissari, S.E. Baumeister, F.F. Demarco, G.G. Nascimento
Individuals of lower socioeconomic position (SEP) experience a greater rate of alcohol-related harms, yet they consume equal or lower amounts of alcohol than higher-SEP individuals. This phenomenon, called the “alcohol harm paradox” (AHP), gained attention recently, and different mechanisms have been proposed to explain it. Since both SEP and alcohol have been suggested to be associated with periodontitis
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Microstructural, Micromechanical Atlas of the Temporomandibular Joint Disc J. Dent. Res. (IF 7.6) Pub Date : 2024-04-10 N. Jiang, P. Tan, Y. Sun, J. Zhou, R. Ren, Z. Li, S. Zhu
The temporomandibular joint (TMJ) disc is mainly composed of collagen, with its arrangement responding to efficient stress distribution. However, microstructural and micromechanical transformations of the TMJ disc under resting, functional, and pathological conditions remain unclear. To address this, our study presents a high-resolution microstructural and mechanical atlas of the porcine TMJ disc.
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A Polygenic Score Predicts Caries Experience in Elderly Swedish Adults J. Dent. Res. (IF 7.6) Pub Date : 2024-04-08 N. Fries, S. Haworth, J.R. Shaffer, A. Esberg, K. Divaris, M.L. Marazita, I. Johansson
Caries is a partially heritable disease, raising the possibility that a polygenic score (PS, a summary of an individual’s genetic propensity for disease) might be a useful tool for risk assessment. To date, PS for some diseases have shown clinical utility, although no PS for caries has been evaluated. The objective of the study was to test whether a PS for caries is associated with disease experience
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Oral–Gut–Estrobolome Axis May Exert a Selective Impact on Oral Cancer J. Dent. Res. (IF 7.6) Pub Date : 2024-04-08 M. Tatullo, J. Nor, G. Orrù, A. Piattelli, E. Cascardi, G. Spagnuolo
A subset of bacterial species that holds genes encoding for β-glucuronidase and β-galactosidase, enzymes involved in the metabolism of conjugated estrogens, is called the “estrobolome.” There is an emerging interest embracing this concept, as it may exert a selective impact on a number of pathologies, including oral cancer. Although the estrobolome bacteria are typically part of the gut microbiota
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UiO-66/AgNPs Coating for Dental Implants in Preventing Bacterial Infections J. Dent. Res. (IF 7.6) Pub Date : 2024-04-06 C. Yu, Y. Yu, Y. Lu, K. Quan, Z. Mao, Y. Zheng, L. Qin, D. Xia
Titanium (Ti)–based biomaterials lack inherent antimicrobial activities, and the dental plaque formed on the implant surface is one of the main risk factors for implant infections. Construction of an antibacterial surface can effectively prevent implant infections and enhance implant success. Silver nanoparticles (AgNPs) exhibit broad antibacterial activity and a low tendency to induce drug resistance
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Zinc- and Fluoride-Releasing Bioactive Glass as a Novel Bone Substitute J. Dent. Res. (IF 7.6) Pub Date : 2024-04-06 T. Kondo, K. Otake, H. Kakinuma, Y. Sato, S. Ambo, H. Egusa
Bioglass 45S5, a silica-based glass, has pioneered a new field of biomaterials. Bioglass 45S5 promotes mineralization through calcium ion release and is widely used in the dental field, including toothpaste formulations. However, the use of Bioglass 45S5 for bone grafting is limited owing to the induction of inflammation, as well as reduced degradation and ion release. Phosphate-based glasses exhibit
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High-Performance Dental Resins Containing a Starburst Monomer J. Dent. Res. (IF 7.6) Pub Date : 2024-03-29 S.Q. Dai, W. Zhou, L.Y. Duan, K. Tang, Z.Y. Yang, R.J. Cao, F.R. Tay, L.N. Niu, J.H. Chen
Dimethacrylate-based chemistries feature extensively as resin monomers in dental resin–based materials due to their distinguished overall performance. However, challenges endure, encompassing inadequate mechanical attributes, volumetric shrinkage, and estrogenicity. Herein, we first synthesized a novel resin monomer, 9-armed starburst polyurethane acrylate (NPUA), via the grafting-onto approach. Compared
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Heterogeneous Association of Tooth Loss with Functional Limitations J. Dent. Res. (IF 7.6) Pub Date : 2024-03-27 Y. Matsuyama, J. Aida, K. Kondo, K. Shiba
Tooth loss is prevalent in older adults and associated with functional capacity decline. Studies on the susceptibility of some individuals to the effects of tooth loss are lacking. This study aimed to investigate the heterogeneity of the association between tooth loss and higher-level functional capacity in older Japanese individuals employing a machine learning approach. This is a prospective cohort
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Social Disadvantage and Multimorbidity Including Oral Conditions in the United States J. Dent. Res. (IF 7.6) Pub Date : 2024-03-20 A. Mirza, R.G. Watt, A. Heilmann, M. Stennett, A. Singh
Existing studies on multimorbidity have largely excluded oral diseases in multimorbidity prevalence estimates. The reason behind this is somewhat unclear, as chronic oral conditions are highly prevalent, affecting over half the global population. To address this gap, we examined the relationship between social disadvantage and multimorbidity, stratifying by the inclusion and exclusion of oral conditions
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AZGP1 Aggravates Macrophage M1 Polarization and Pyroptosis in Periodontitis J. Dent. Res. (IF 7.6) Pub Date : 2024-03-16 S. Yang, Y. Yin, Y. Sun, D. Ai, X. Xia, X. Xu, J. Song
Periodontal tissue destruction in periodontitis is a consequence of the host inflammatory response to periodontal pathogens, which could be aggravated in the presence of type 2 diabetes mellitus (T2DM). Accumulating evidence highlights the intricate involvement of macrophage-mediated inflammation in the pathogenesis of periodontitis under both normal and T2DM conditions. However, the underlying mechanism
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Neighborhood Deprivation and Changes in Oral Health in Older Age: A Longitudinal Population-Based Study J. Dent. Res. (IF 7.6) Pub Date : 2024-02-28 S.G. Ganbavale, E. Papachristou, J.C. Mathers, A.O. Papacosta, L.T. Lennon, P.H. Whincup, S.G. Wannamethee, S.E. Ramsay
The aim of this study was to examine the extent to which neighborhood-level socioeconomic factors (objective and perceived) are associated with poor oral health in older adults over time, independent of individual socioeconomic position. Data for this cross-sectional and longitudinal observation study came from a socially and geographically representative cohort of men aged 71 to 92 y in 2010–12 (
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IL-36 Regulates Neutrophil Chemotaxis and Bone Loss at the Oral Barrier J. Dent. Res. (IF 7.6) Pub Date : 2024-02-28 J. Liu, H. Meng, Y. Mao, L. Zhong, W. Pan, Q. Chen
Tissue-specific mechanisms regulate neutrophil immunity at the oral barrier, which plays a key role in periodontitis. Although it has been proposed that fibroblasts emit a powerful neutrophil chemotactic signal, how this chemotactic signal is driven has not been clear. The objective of this study was to investigate the site-specific regulatory mechanisms by which fibroblasts drive powerful neutrophil
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Injectable Tissue-Specific Hydrogel System for Pulp–Dentin Regeneration J. Dent. Res. (IF 7.6) Pub Date : 2024-02-27 Y. Han, J. Xu, H. Chopra, Z. Zhang, N. Dubey, W.L. Dissanayaka, J.E. Nör, M.C. Bottino
The quest for finding a suitable scaffold system that supports cell survival and function and, ultimately, the regeneration of the pulp–dentin complex remains challenging. Herein, we hypothesized that dental pulp stem cells (DPSCs) encapsulated in a collagen-based hydrogel with varying stiffness would regenerate functional dental pulp and dentin when concentrically injected into the tooth slices. Collagen
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Exploring Recent Decreases in First Molar Sealants among US Children J. Dent. Res. (IF 7.6) Pub Date : 2024-02-27 M. Lin, S. O. Griffin, C. H. Li, L. Wei, L. Espinoza, C. Y. Wang, G. Thornton-Evans
Analyses of National Health and Nutrition Examination Survey (NHANES) data suggested a significant decrease in sealant prevalence among children between 2011 to 2014 and 2015 to 2018. We explore whether this decrease could be associated with possible changes in 1) clinical sealant delivery, 2) dental materials (i.e., increased use of glass ionomer [GI] sealants resulting in an inability to detect sealant
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A Bilayer Method for Measuring Toughness and Strength of Dental Ceramics J. Dent. Res. (IF 7.6) Pub Date : 2024-02-27 H. Chai, J. Russ, S. Vardhaman, C.H. Lim, Y. Zhang
The ever-increasing usage of ceramic materials in restorative dentistry necessitates a simple and effective method to evaluate flexural strength σF and fracture toughness KC. We propose a novel method to determine these quantities using a bilayer specimen composed of a brittle plate adhesively bonded onto a transparent polycarbonate substrate. When this bilayer structure is placed under spherical indentation
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Single-Cell Transcriptomic Analysis of Salivary Gland Endothelial Cells J. Dent. Res. (IF 7.6) Pub Date : 2024-02-27 A.L. Altrieth, J. Kenney, D.A. Nelson, E.G. Suarez, V. Gellatly, S. Gabunia, M. Larsen
Vascular endothelial cells have important tissue-specific functions in fibrosis and regeneration. In the salivary gland, endothelial cells are required for proper development, but their roles within adult glands are largely unknown. To identify ligand–receptor interactions between endothelial cells and other cell types that may be important during fibrosis and regeneration, we used a reversible ductal
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The Essential Role of Proteoglycans and Glycosaminoglycans in Odontogenesis J. Dent. Res. (IF 7.6) Pub Date : 2024-02-26 J. Chen, T. Sun, B. Lin, B. Wu, J. Wu
Tooth development and regeneration are regulated through a complex signaling network. Previous studies have focused on the exploration of intracellular signaling regulatory networks, but the regulatory roles of extracellular networks have only been revealed recently. Proteoglycans, which are essential components of the extracellular matrix (ECM) and pivotal signaling molecules, are extensively involved
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Response to Letter, “Autophagy Plays a Crucial Role in Ameloblast Differentiation” J. Dent. Res. (IF 7.6) Pub Date : 2024-02-21 C. Iwaya, J. Iwata
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Biological Properties of 3D-Printed Zirconia Implants with p-Cell Structures J. Dent. Res. (IF 7.6) Pub Date : 2024-02-20 W. Qin, MH. Shen, N. Gan, BH. Xing, J. Sun, Z. Zhao, T. Jiao
Research on 3-dimensional (3D) printed porous zirconia-based dental implants is still in its infancy. This study aimed to evaluate the biological responses of novel zirconia implants with p-cell structures fabricated by 3D printing. The solid zirconia samples exhibited comparable density, 3-point flexural strength, and accelerated aging properties compared to specimens prepared previously by conventional
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Intelligently Quantifying the Entire Irregular Dental Structure J. Dent. Res. (IF 7.6) Pub Date : 2024-02-19 H. Liu, J. Duan, P. Zeng, M. Shi, J. Zeng, S. Chen, Z. Gong, Z. Chen, J. Qin, Z. Chen
Quantitative analysis of irregular anatomical structures is crucial in oral medicine, but clinicians often typically measure only several representative indicators within the structure as references. Deep learning semantic segmentation offers the potential for entire quantitative analysis. However, challenges persist, including segmentation difficulties due to unclear boundaries and acquiring measurement
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Effect of Periodontitis and Periodontal Therapy on Oral and Gut Microbiota J. Dent. Res. (IF 7.6) Pub Date : 2024-02-16 G. Baima, I. Ferrocino, V. Del Lupo, E. Colonna, V. Thumbigere-Math, G.P. Caviglia, I. Franciosa, G.M. Mariani, M. Romandini, D.G. Ribaldone, F. Romano, M. Aimetti
Mounting evidence indicates that periodontitis-related oral bacteria may contribute to gut microbial dysbiosis. This clinical study aimed to explore the oral–gut microbial signatures associated with periodontitis and to longitudinally evaluate the effect of periodontal treatment on the oral and gut microbial composition. Stool and saliva samples from generalized stage III/IV periodontitis patients
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Salivary Glands and Viral Pathogenesis J. Dent. Res. (IF 7.6) Pub Date : 2024-02-12 N. Atyeo, J.O. Maldonado, B.M. Warner, J.A. Chiorini
The oral cavity is an epidemiologically relevant route of viral transmission due to the shedding of viruses in saliva. With advancements in salivary diagnostics, an increasing number of viruses have been detected. However, the anatomic source of virus in saliva is still largely unknown. Some viruses have a well-established tropism for the salivary glands (SGs), and recent studies have emphasized the
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Murine IRF8 Mutation Offers New Insight into Osteoclast and Root Resorption J. Dent. Res. (IF 7.6) Pub Date : 2024-02-12 A. Das, S.K. Yesupatham, D. Allison, H. Tanwar, J. Gnanasekaran, B. Kear, X. Wang, S. Wang, C. Zachariadou, Y. Abbasi, M.K. Chung, K. Ozato, C. Liu, B.L. Foster, V. Thumbigere-Math
Interferon regulatory factor 8 (IRF8), a transcription factor expressed in immune cells, functions as a negative regulator of osteoclasts and helps maintain dental and skeletal homeostasis. Previously, we reported that a novel mutation in the IRF8 gene increases susceptibility to multiple idiopathic cervical root resorption (MICRR), a form of tooth root resorption mediated by increased osteoclast activity
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P. gingivalis–Induced TLR2 Interactome Analysis Reveals Association with PARP9 J. Dent. Res. (IF 7.6) Pub Date : 2024-02-12 K. Pandi, S. Angabo, H. Makkawi, H. Benyamini, S. Elgavish, G. Nussbaum
Porphyromonas gingivalis is a Gram-negative anaerobic bacterium strongly associated with periodontal disease. Toll-like receptor 2 (TLR2) is indispensable for the host response to P. gingivalis, but P. gingivalis escapes from immune clearance via TLR2-dependent activation of phosphoinositide-3-kinase (PI3K). To probe the TLR2-dependent escape pathway of P. gingivalis, we analyzed the TLR2 interactome
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In Situ Photo-Crosslinkable Protein Bioadhesive for Bone Graft Fixation J. Dent. Res. (IF 7.6) Pub Date : 2024-02-06 J. Yun, I.H. Nam, H. Lee, Y.K. Jo, H. Lee, S.H. Jun, H.J. Cha
Bone grafting is a fundamental dental surgery procedure widely used for implant placement and periodontal disease management treatments. Despite its broad applications, vertical bone augmentation presents unique challenges, including the risk of graft displacement due to gravitational and masticatory forces. Traditional physical stabilization methods introduce additional complexities and risks, underscoring
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Gli1+ Periodontal Mesenchymal Stem Cells in Periodontitis J. Dent. Res. (IF 7.6) Pub Date : 2024-01-29 Y. Deng, Q. Li, K.K.H. Svoboda, L.A. Opperman, L.B. Ruest, X. Liu
Periodontal mesenchymal stem cells (MSCs) play a crucial role in maintaining periodontium homeostasis and in tissue repair. However, little is known about how periodontal MSCs in vivo respond under periodontal disease conditions, posing a challenge for periodontium tissue regeneration. In this study, Gli1 was used as a periodontal MSC marker and combined with a Gli1-cre ERT2 mouse model for lineage
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Sjögren’s Disease and Oral Health: A Genetic Instrumental Variable Analysis J. Dent. Res. (IF 7.6) Pub Date : 2024-01-29 S.L. Reckelkamm, Z. Alayash, B. Holtfreter, M. Nolde, S.E. Baumeister
Epidemiological studies have consistently shown that Sjögren’s disease (SjD) increases the risk of dental caries. Despite similar evidence indicating an elevated risk of periodontitis, SjD remains a disputed risk factor for this disease. The risk of bias in observational research is a major impediment to confirming this link. Within an instrumental variable framework, genetic variants associated with
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Composite Containing Calcium Phosphate Particles Functionalized with 10-MDP J. Dent. Res. (IF 7.6) Pub Date : 2024-01-29 R.A.A. da Silva, R.B. Trinca, H.S. Vilela, R.R. Braga
The phosphate ester monomer 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP) is capable of bonding to hydroxyapatite and, for this reason, is a key component of several self-etch adhesives. In this study, dicalcium phosphate dihydrate particles (DCPD; CaHPO4.2H2O) were functionalized with 10-MDP and used to formulate an experimental composite with 50 vol% inorganic content (3:1 DCPD:silanated
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Pth1r in Neural Crest Cells Regulates Nasal Cartilage Differentiation J. Dent. Res. (IF 7.6) Pub Date : 2024-01-18 K. Amano, D. Okuzaki, Y. Kitaoka, S. Kato, M. Fujiwara, S. Tanaka, S. Iida
Neural crest cells (NCC) arise from the dorsal margin of the neural plate border and comprise a unique cell population that migrates to and creates the craniofacial region. Although factors including Shh, Fgf8, and bone morphogenetic proteins have been shown to regulate these biological events, the role of parathyroid hormone 1 receptor (Pth1r) has been less studied. We generated an NCC-specific mouse
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Copy Number Alterations Predict Development of OSCC from Oral Leukoplakia J. Dent. Res. (IF 7.6) Pub Date : 2024-01-13 X. Cai, J. Zhang, L. Li, L. Liu, M. Tang, X. Zhou, C. Peng, X. Li, X. Chen, M. Xu, H. Zhang, J. Wang, Y. Huang, T. Li
Oral leukoplakia (OLK) is a common type of potentially malignant disorder. Early identification of the malignancy potential leads to a better management of OLK and prediction of development of oral squamous cell carcinoma (OSCC). However, there has been no effective biomarker to assess the risk of malignancy in OLK. Genomic copy number alteration (CNA) is a complex chromosomal structural variation
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Casp11 Deficiency Alters Subgingival Microbiota and Attenuates Periodontitis J. Dent. Res. (IF 7.6) Pub Date : 2024-01-10 S.L. Fu, Y.Y. Qian, A.N. Dai, H.Y. Li, X.H. Jin, W.T. He, S. Kang, P.H. Ding
Periodontitis (PD) is the primary cause of tooth loss in adults. Porphyromonas gingivalis ( P.g), a keystone pathogen, has been identified as a crucial contributor to this process. Pyroptosis activation in PD is acknowledged, with accumulating evidence underscoring the crucial role of Caspase-11 (described as Caspase-4/5 in humans)–mediated noncanonical pyroptosis. However, the mechanism behind its
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Lacticaseibacillus rhamnosus GG Improves Periodontal Bone Repair via Gut–Blood Axis in Hyperlipidemia J. Dent. Res. (IF 7.6) Pub Date : 2024-01-10 Y. Huang, R. Ge, J. Qian, J. Lu, D. Qiao, R. Chen, H. Jiang, D. Cui, T. Zhang, N. Wang, S. He, M. Wang, F. Yan
Periodontal bone regeneration remains a clinical challenge, and hyperlipidemia can aggravate alveolar bone resorption. Probiotics have recently been reported to improve bone mass. We aimed to determine the role of Lacticaseibacillus rhamnosus GG (LGG) in periodontal bone regeneration improvement within the context of periodontitis with hyperlipidemia. A Sprague Dawley rat model for periodontitis, hyperlipidemia
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Impact of Periodontitis on the Leakage of Oral Bacteria to the Gut J. Dent. Res. (IF 7.6) Pub Date : 2024-01-09 E. Buetas, M. Jordán-López, A. López-Roldán, A. Mira, M. Carda-Diéguez
Colorectal cancer (CRC) and periodontitis have recently been related due to the higher incidence of CRC in periodontal patients and the involvement of periodontal pathogens in carcinogenesis, suggesting that leakage from the oral cavity to the gut occurs. However, the magnitude of this pass-through in healthy individuals is controversial, and the effect that periodontitis could play in it is understudied
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Dual Role of IGF2BP2 in Osteoimmunomodulation during Periodontitis J. Dent. Res. (IF 7.6) Pub Date : 2024-01-09 X.X. Ma, X.Y. Zhou, M.G. Feng, Y.T. Ji, F.F. Song, Q.C. Tang, Q. He, Y.F. Zhang
Periodontitis is a complex disease characterized by distinct inflammatory stages, with a peak of inflammation in the early phase and less prominent inflammation in the advanced phase. The insulin-like growth factor 2-binding proteins 2 (IGF2BP2) has recently been identified as a new m6A reader that protects m6A-modified messenger RNAs (mRNAs) from decay, thus participating in multiple biological processes
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Electrolyzed Saline Targets Biofilm Periodontal Pathogens In Vitro J. Dent. Res. (IF 7.6) Pub Date : 2024-01-08 N. Zayed, H. Munjaković, M.K. Aktan, K. Simoens, K. Bernaerts, N. Boon, A. Braem, F. Pamuk, M. Saghi, W. Van Holm, A. Fidler, R. Gašperšič, W. Teughels
Preventing the development and recurrence of periodontal diseases often includes antimicrobial mouthrinses to control the growth of the periodontal pathogens. Most antimicrobials are nonselective, targeting the symbiotic oral species as well as the dysbiosis-inducing ones. This affects the overall microbial composition and metabolic activity and consequently the host–microbe interactions, which can
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Intestinal Translocation of Live Porphyromonas gingivalis Drives Insulin Resistance J. Dent. Res. (IF 7.6) Pub Date : 2024-01-08 C. Niu, W. Lv, X. Zhu, Z. Dong, K. Yuan, Q. Jin, P. Zhang, P. Li, M. Mao, T. Dong, Z. Chen, J. Luo, L. Hou, C. Zhang, K. Hao, S. Chen, Z. Huang
Periodontitis has been emphasized as a risk factor of insulin resistance–related systemic diseases. Accumulating evidence has suggested a possible “oral–gut axis” linking oral infection and extraoral diseases, but it remains unclear whether periodontal pathogens can survive the barriers of the digestive tract and how they play their pathogenic roles. The present study established a periodontitis mouse
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Redefining Mucosal Inflammation with Spatial Genomics J. Dent. Res. (IF 7.6) Pub Date : 2024-01-03 A.J. Caetano, P.T. Sharpe
The human oral mucosa contains one of the most complex cellular systems that are essential for normal physiology and defense against a wide variety of local pathogens. Evolving techniques and experimental systems have helped refine our understanding of this complex cellular network. Current single-cell RNA sequencing methods can resolve subtle differences between cell types and states, thus providing
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Rapid Intrafibrillar Mineralization Strategy Enhances Adhesive-Dentin Interface. J. Dent. Res. (IF 7.6) Pub Date : 2023-11-22 X Gao,Z Wang,H Yang,C Huang
Biomimetic mineralization of dentin collagen appears to be a promising strategy to optimize dentin bonding durability. However, traditional postbonding mineralization strategies based on Ca/P ion release still have some drawbacks, such as being time-consuming, having a spatiotemporal mismatch, and having limited intrafibrillar minerals. To tackle these problems, a prebonding rapid intrafibrillar mineralization
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ASH2L, Core Subunit of H3K4 Methylation Complex, Regulates Amelogenesis. J. Dent. Res. (IF 7.6) Pub Date : 2023-11-21 X Zhu,Z Ma,F Xie,J Wang
Histone methylation assumes a crucial role in the intricate process of enamel development. Our study has illuminated the substantial prevalence of H3K4me3 distribution, spanning from the cap stage to the late bell stage of dental germs. In order to delve into the role of H3K4me3 modification in amelogenesis and unravel the underlying mechanisms, we performed a conditional knockout of Ash2l, a core
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Single-Cell Transcriptomic Analysis of Dental Pulp and Periodontal Ligament Stem Cells. J. Dent. Res. (IF 7.6) Pub Date : 2023-11-20 Y Yang,T Alves,M Z Miao,Y C Wu,G Li,J Lou,H Hasturk,T E Van Dyke,A Kantarci,D Wu
The regeneration of periodontal, periapical, and pulpal tissues is a complex process requiring the direct involvement of cells derived from pluripotent stem cells in the periodontal ligament and dental pulp. Dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) are spatially distinct with the potential to differentiate into similar functional and phenotypic cells. We aimed to
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Letter to the Editor, "Autophagy Plays a Crucial Role in Ameloblast Differentiation". J. Dent. Res. (IF 7.6) Pub Date : 2023-11-15 S Sukseree,R Gruber,E Tschachler,L Eckhart
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Endochondral Repair of Jawbone Defects Using Periosteal Cell Spheroids. J. Dent. Res. (IF 7.6) Pub Date : 2023-11-15 J Zhu,S Zhang,S Jin,C Huang,B Shi,Z Chen,W Ji
Recapitulation of the natural healing process is receiving increasing recognition as a strategy to induce robust tissue regeneration. Endochondral ossification has been recognized as an essential reparative approach in natural jawbone defect healing. However, such an approach has been overlooked in the recent development of cell-based therapeutics for jawbone repair. Therefore, this study aimed to
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Sex-Related Effect of Aging in Gingival Gamma-Delta T Cells. J. Dent. Res. (IF 7.6) Pub Date : 2023-11-15 V Tubero Euzebio Alves,M E C Bruno,S Mukherjee,L Wang,R J Danaher,L Su,M E Starr,O A Gonzalez
Aging affects the number and function of gamma-delta (γδ) T cells in a tissue-specific manner, modifying the risk for inflammatory disease. These aging-related γδT-cell variations in gingival tissues that could increase the risk for inflammation and periodontal disease remain unknown. Here we sought to identify quantitative and qualitative variations in gingival γδT cells associated with aging that
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Clinically Oriented CBCT Periapical Lesion Evaluation via 3D CNN Algorithm. J. Dent. Res. (IF 7.6) Pub Date : 2023-11-15 W T Fu,Q K Zhu,N Li,Y Q Wang,S L Deng,H P Chen,J Shen,L Y Meng,Z Bian
Apical periodontitis (AP) is one of the most prevalent disorders in dentistry. However, it can be underdiagnosed in asymptomatic patients. In addition, the perioperative evaluation of 3-dimensional (3D) lesion volume is of great clinical relevance, but the required slice-by-slice manual delineation method is time- and labor-intensive. Here, for quickly and accurately detecting and segmenting periapical
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Systemic Metabolic Signatures of Oral Diseases. J. Dent. Res. (IF 7.6) Pub Date : 2023-11-15 A Salminen,A M Määttä,P Mäntylä,J Leskelä,M Pietiäinen,K Buhlin,A L Suominen,S Paju,W Sattler,J Sinisalo,P J Pussinen
Systemic metabolic signatures of oral diseases have been rarely investigated, and prospective studies do not exist. We analyzed whether signs of current or past infectious/inflammatory oral diseases are associated with circulating metabolites. Two study populations were included: the population-based Health-2000 (n = 6,229) and Parogene (n = 452), a cohort of patients with an indication to coronary
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Sex as a Biological Variable in Oral Diseases: Evidence and Future Prospects. J. Dent. Res. (IF 7.6) Pub Date : 2023-11-15 L Sangalli,L C Souza,A Letra,L Shaddox,E Ioannidou
The interest of the scientific community on sex and gender differences in health and disease has increased substantially over the past 25 to 30 y as a result of a long process of events and policies in the biomedical field. This is crucial as compelling evidence from human and animal model studies has demonstrated that sex and gender influence health, molecular and cellular processes, and response
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Enhanced Bonding to Caries-Affected Dentin Using an Isocyanate-Based Primer. J. Dent. Res. (IF 7.6) Pub Date : 2023-11-10 K Tang,F Wang,S Q Dai,Z Y Yang,L Y Duan,M L Luo,F R Tay,L N Niu,W Zhou,J H Chen
Dental caries is the most common oral disease and the most common cause of resin restorations. In minimally invasive dentistry, the principle behind cavity preparation is to remove external caries-infected dentin (CID) and preserve internal caries-affected dentin (CAD) and sound dentin (SD). The cavity floor is mainly composed of CAD, but the poor bonding performance of CAD has become a widespread
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Epigenetic Regulation of Ameloblast Differentiation by HMGN Proteins. J. Dent. Res. (IF 7.6) Pub Date : 2023-11-10 B He,V Kram,T Furusawa,O Duverger,E Y Chu,R Nanduri,M Ishikawa,P Zhang,B A Amendt,J S Lee,M Bustin
Dental enamel formation is coordinated by ameloblast differentiation, production of enamel matrix proteins, and crystal growth. The factors regulating ameloblast differentiation are not fully understood. Here we show that the high mobility group N (HMGN) nucleosomal binding proteins modulate the rate of ameloblast differentiation and enamel formation. We found that HMGN1 and HMGN2 proteins are downregulated
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Preinterventional Third-Molar Assessment Using Robust Machine Learning. J. Dent. Res. (IF 7.6) Pub Date : 2023-11-09 J S Carvalho,M Lotz,L Rubi,S Unger,T Pfister,J M Buhmann,B Stadlinger
Machine learning (ML) models, especially deep neural networks, are increasingly being used for the analysis of medical images and as a supporting tool for clinical decision-making. In this study, we propose an artificial intelligence system to facilitate dental decision-making for the removal of mandibular third molars (M3M) based on 2-dimensional orthopantograms and the risk assessment of such a procedure
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Gestational Serum Retinol Deficiency Is Associated with Enamel Hypoplasia. J. Dent. Res. (IF 7.6) Pub Date : 2023-10-29 S R Samuel,C W M Lai,M M Khan,M G Mathew,M S Kramer,C-Y S Hsu
Enamel hypoplasia (EH) is a prevalent developmental defect of teeth that can result from various insults, including prenatal nutrient deficiencies. This study aimed to evaluate the association between prenatal serum retinol deficiency and EH in the deciduous teeth of offspring at 2-y of age. A cohort of 1,450 pregnant women was enrolled, and their prenatal nutritional status was assessed between 12
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Triple Function of Amelogenin Peptide-Chitosan Hydrogel for Dentin Repair. J. Dent. Res. (IF 7.6) Pub Date : 2023-10-26 J Cai,J Moradian-Oldak
Biomimetic strategies like peptide-guided collagen mineralization promise to enhance the effectiveness of dentin remineralization. We recently reported that rationally designed amelogenin-derived peptides P26 and P32 promoted apatite nucleation, mineralized collagen, and showed potential in enamel regrowth and dentin remineralization. To facilitate the clinical application of amelogenin-derived peptides
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Circulating Metabolites and Dental Traits: A Mendelian Randomization Study. J. Dent. Res. (IF 7.6) Pub Date : 2023-10-21 Q Zheng,W Li,Y Zhang,X Liu,Y Fu,S Luo,X Deng,C Zeng
It is of great importance to uncover causal biomarkers to gain insight into the pathogenesis of oral diseases and identify novel treatment targets for prevention and treatment thereof. This study aimed to systematically evaluate the causal effects of hundreds of metabolites on 10 dental traits using a 2-sample Mendelian randomization (MR) approach. Genetic variants from genome-wide association studies
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Designing Calcium Silicate Cements with On-Demand Properties for Precision Endodontics. J. Dent. Res. (IF 7.6) Pub Date : 2023-10-20 A Cahyanto,P Rath,T X Teo,S S Tong,R Malhotra,B N Cavalcanti,L Z Lim,K S Min,D Ho,W F Lu,V Rosa
Calcium silicate (C3S) cements are available in kits that do not account for patients' specific needs or clinicians' preferences regarding setting time, radiopacity, mechanical, and handling properties. Moreover, slight variations in powder components and liquid content affect cement's properties and bioactivity. Unfortunately, it is virtually impossible to optimize several cement properties simultaneously
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RvE1 Promotes Axin2+ Cell Regeneration and Reduces Bacterial Invasion. J. Dent. Res. (IF 7.6) Pub Date : 2023-10-13 Y-C Wu,N Yu,C A Rivas,N Mehrnia,A Kantarci,T E Van Dyke
Vital pulp therapy and root canal therapy (RCT) are the dominant treatment for irreversible pulpitis. While the success rate of these procedures is favorable, they have some limitations. For instance, RCT leads to removing significant dentin in the coronal third of the tooth that increases root-fracture risk, which forces tooth removal. The ideal therapeutic goal is dental pulp regeneration, which
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miRNAs from Inflamed Gingiva Link Gene Signaling to Increased MET Expression. J. Dent. Res. (IF 7.6) Pub Date : 2023-10-11 L Zheng,A Chopra,J Weiner,D Beule,H Dommisch,A S Schaefer
Several array-based microRNA (miRNA) expression studies independently showed increased expression of miRNAs hsa-miR-130a-3p, -142-3p, -144-3p, -144-5p, -223-3p, -17-5p, and -30e-5p in gingiva affected by periodontal inflammation. We aimed to determine direct target genes and signaling pathways regulated by these miRNAs to identify processes relevant to gingival inflammatory responses and tissue homeostasis
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Circ-Slain2 Alleviates Cartilage Degradation and Inflammation of TMJOA. J. Dent. Res. (IF 7.6) Pub Date : 2023-10-10 X Pan,Z Zhao,X Huang,X Cen
Temporomandibular joint osteoarthritis (TMJOA) is a degenerative disease with the cessation of matrix anabolism and aggravation of inflammation, which results in severe pain and impaired joint function. However, the mechanisms are not well understood. Circular RNAs (circRNAs) are reported to have various biological functions and participate in the development, diagnosis, prognosis, and treatment of